| 摘要: | 最近以中草藥或健康食品來治療或預防疾病的觀念則越來越受到重視。2011年糖尿病已居臺灣十大死因的第四位，且第二型糖尿病占國人糖尿病人口的95％左右，因此探討防風通聖散(Bofutsushosan; BOF)及大柴胡湯(Daisaikoto; DAI)兩種傳統中草藥方劑對國人保健應有其重要的意義。民間多認為防風通聖散(BOF)及大柴胡湯(DAI)含有19種及8種藥材組合而成，具有保肝、減肥及降血脂等效能。但是防風通聖散(BOF)及大柴胡湯(DAI)兩種方劑對抗第二型糖尿病的活性與其保健功能目前並不明瞭，因此值得深入研究。本研究主要研究目的，包括：第一年計畫以細胞模式探討BOF及DAI與其活性成分對小鼠骨骼肌(C2C12)細胞之細胞存活率、葡萄糖吸收效力及其對胰島素訊息、GLUT-4路徑及對AMPK訊息路徑等進行探討。另外。以第二型糖尿病動物模式，使用高脂肪飲食加入防風通聖散(BOF)及大柴胡湯(DAI)95%乙醇萃取物對改善葡萄糖吸收功能及胰島素阻抗、GLUT-4路徑及AMPK路徑等之相關保健功效及分子機制進行研究。研究結果發現25~50 μg/ml的防風通聖散(BOF)及大柴胡湯(DAI)的95%乙醇萃取物對C2C12細胞能有效的促進細胞增生，並刺激葡萄糖吸收效力(p<0.05)。以HPLC分析法測定，發現防風通聖散(BOF)及大柴胡湯(DAI)兩方劑的95%乙醇萃取物主要之生物活性成分為baicalin。50 μg/ml防風通聖散(BOF)及大柴胡湯(DAI)的95%乙醇萃取物均會明顯提高活化及磷酸化IRS-1、活化及磷酸化ACC及磷酸化AMPK蛋白質之表現，並促進GLUT-4蛋白質之表現，可能與脂肪代謝有相關性。大柴胡湯(DAI)的95%乙醇萃取物會增加C2C12細胞之磷酸化p38及ERK蛋白質之表現。防風通聖散(BOF)及大柴胡湯(DAI)的95%乙醇萃取物之第二型糖尿病動物模式結果發現以防風通聖散(BOF)及大柴胡湯(DAI)的95%乙醇萃取物均具有顯著的降低血糖的功效(p<0.05)，並且對血液胰島素之濃度不影響。
There is growing interest in the use of traditional Chinese herbal medicines and health foods for the treatment and prevention of diseases. In 2011, diabetes mellitus (DM) was the fourth among the top 10 causes of death in Taiwan, and more than 95% above are type 2 DM. In general, Chinese herb medicines, such as Bofutsushosan (BOF; consisting of 19 components) and Daisaikoto (DAI; constituted from 8 ingredients) possess hepatoprotective, anti-obesity, and hypolipidemic activities among other therapeutic properties. However, their anti-diabetic effects remain unclear, which warrant research investigation. The aims of this study comprise of the following: for the first year, using in vitro cell model including C2C12 cells, we will examine the cell viability and, under type 2 diabetic condition, their mechanism(s) of therapeutic effects; furthermore, in vivo high fat-diet animal model will be used to examine the effects of BOF and DAI as well as their active compounds to elucidate their functional properties of glucose absorption, insulin resistance, GLUT-4, as well as AMPK pathways. Results displayed that the effectively increased the proliferation and stimulated glucose-uptake from 25 to 50 μg/ml BOF and DAI 95% ethanol extracts in the C2C12 cells. The HPLC analysis method has been determined the major biologically active ingredient, namely baicalin of the BOF and DAI 95% ethanol extracts. 50 μg/ml BOF and DAI 95% ethanol extracts significantly up-regulated activation and phosphorylation of (IRS-1 and ACC), and phosphorylation of AMPK proteins, and enhanced the expression of GLUT-4, might be correlated with the lipid metabolism. In addition, DAI 95% ethanol extract markedly increased phosphorylation of p38 and ERK in C2C12 cells. Both BOF and DAI 95% ethanol extracts significantly decreased blood glucose levels (p<0.05) and insulin decrease was not apparent in the type 2 diabetic animal model. |
