To review the statistics of occupation accident occurring in the Southern Taiwan Science Park, There are16.2% of occupation accident caused from machinery maintenanceThe main injury types including rolls, cuts and crushed, in particular, occur in abnormality excluded, maintenance, testing-maintenance. The injured parts including limbs, head by the machine crush, crushed, drawn in have the highest risk. The main causes of these accidents are lack of safety guarding or safety device failure. The secondry causes are operation errors. The aim of this study is to explore the mechanical injury occurring in photovoltaic opto-electrical LED manufacturer for process maintenance operations due to contact with the original materials, chemical substances and mechanical residual energy of two mainly hazard factors. By uses of risk identification, questionnaire and field survey visit. The study found that a large number of chemical products gases and toxic substance were used in front / middle of the LED manufacturer process. It is the main risk for exposing to residues. For decreasing the risk of exposure of the residues, the maintenance staff can prolong the machine decontamination time, by using machine external local exhaust facilities and wearing appropriate and effective personal protection equipment. The characterist of the machine functions is simple, the mainly physical risk of harmful residual and kinetic energy. To implement the power-off operations, and strengthen the nature of the machine's safety interlock mechanism can reduce the incidence of occupational hazards. The study suggests that the initial design of machine need accordind to the CE, SEMI-related norms, and strengthen intrinsic safety of machine and enforce maintenance operating system. In order to construct safe maintenance and operation environment, proper planning for the area of operation and pre-ranked human, with the equipment to the scene support measures are also needed.