系列化合物之抗氧化、抗發炎及DNA修復之機制探討

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Title:	系列化合物之抗氧化、抗發炎及DNA修復之機制探討 The mechanisms of compounds on antioxidant, anti-inflammatory and DNA repair activities
Authors:	陳品儒
Contributors:	化妝品科技研究所梁家華
Keywords:	抗氧化抗發炎 DNA修復 anti-inflammatory antioxidant DNA repair
Date:	2012
Issue Date:	2012-11-27 11:35:02 (UTC+8)
Abstract:	(Part 1)隨著不同濃度的P作用下，正常細胞和癌細胞的存活度及細胞內ROS含量有不同的趨勢，此結果與細胞中抗氧化酵素過氧化氫酶(catalase)、穀胱甘肽過氧化酶(glutathione peroxidase,GPx)及超氧化歧化酶(superoxide dismutase, SOD)的活性有關。(Part 2)探討CA衍生物之抗氧化、抗發炎及抑制脂質生成的能力評估。BC 和EBCA具有良好的抗氧化活性且效果比CA、維他命C、rutin和trolox還來的好。BC 和EBCA也可以提高BNL CL.2和HaCaT細胞內抗氧化酵素catalase、SOD 和GPx的活性。而BC and EBCA藉由抑制COX-2、iNOS、TNF-α、IL-1 和IL-6的表現來達到抗發炎的效果。另外，本論文也證實BC 和EBCA藉由調控脂質生成相關因子PPAR-γ、SEBP1-c、FAS、leptin和resistin來達到抑制脂肪細胞分化及脂肪堆積的效果並且可以有效降低肥胖老鼠的體重。因此，本論文證實BC和EBCA具有抗氧化、抗發炎及抑制脂質的能力。(Part 3)利用DBPG進行其對NER修復系統之相關機制。試驗中發現DBPG可以促使正常細胞(HaCaT)和纖維母細胞(Hs68)增生，並且是藉由調節NER系統之相關因子來修復由UV照射引起的DNA之損傷。 (Part 1) In this report, cell viability and intracellular ROS content of cancer cell and normal cell is different by various concentrations P treated. This associated with the regulation of antioxidant enzyme activity such as catalase, glutathione peroxidase (GPx) and superoxide dismutase (SOD). (Part 2) The aims of this study investigated the antioxidant, anti-inflammatory and anti-obesity effects of CA derivative. BC and EBCA had better antioxidant activity than that of CA, ascorbic acid, rutin and trolox. BC and EBCA also increased the antioxidant enzyme activities of catalase, SOD and GPx in BNL CL.2 and HaCaT cells. BC and EBCA exerts anti-inflammatory effects by suppressing COX-2, iNOS, TNF-α, IL-1 and IL-6 expression. Besides, the present study is demonstrate that BC and EBCA was effective in reducing lipid accumulation in adipocytes by regulating adipogenic transcription factors (PPAR-γ and SEBP1-c) and adipocytokine (FAS, leptin and resistin) gene expression. BC and EBCA also decreased body weight in obese mice. The results showed that BC and EBCA might serve as a natural antioxidant, anti-inflammatory and anti-obesity agent. (Part 3) To explore the mechanism of DBPG on NER, the repairs of UV-induced DNAdamages in human keratinocyte cells were investigated. Thecell proliferation was arrested to repair DNA damages inresponse to UV irradiation.
Relation:	校內校外均不公開，學年度：100，80頁
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Dissertations and Theses

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