Studies on the Large Form Urokinase from Human Urine II. Separation and Characterization of Multiple Forms of Large Form Urokinase

Chia Nan University of Pharmacy & Science Institutional Repository > 嘉南學報 > 9 期 (1983) > Item 310902800/25961

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標題:	Studies on the Large Form Urokinase from Human Urine II. Separation and Characterization of Multiple Forms of Large Form Urokinase
作者:	Dong-Bor Yeh Jong-Ching Su
貢獻者:	食品衛生科
日期:	1983
上傳時間:	2012-11-16 09:01:43 (UTC+8)
摘要:	When the purified L-UK was further separated by prepalative isoelectric-focusing electrophoresis, three forms different in pI values (9.9, 8.9 and 8.35) could be obtained. The chemical compositions and some other molecular and kinetic parameters (using benzoyl-L-valyl-glycyl-L-arginine-p-nitroanilide (BVGANA) or plasminogen as substrate) of the multiforms of L-UK were assessed and compared. From these data, several valuable findings were obtained as follows: 1) Although the total number of acidic amino acid residues was 1.1 to 1.2 fold larger than the sum of basic amino acid residues, the high pI values of L-UK forms (8.4 to 9.9) suggested the presence of acidic amino acid in their amide form in a large proportion. 2) The difference in pIs of the different L-UK forms was mainly due to the difference in sialic acid content. 3) Glucose (or sucrose) was found in the resolved L-UK forms but not in the original L-UK unresolved. It indicated that sugar might be adsorbed onto the enzyme molecule in a low ionic strength and high sugar content medium. 4) A sugar content pattem (but not amino acid content) distinctly different from those published by others was obtained. This discrepancy might be due to the difference in the sources of enzyme preparations. 5) The similarity in the kinetic parameters suggested that the different forms of L-UK have a similar affinity toward the substrate uscd (BVGANA and plasminogen).
關聯:	嘉南學報 9, A49 轉載自 Proc. Natl. Sci. Counc. B. ROC, 7(2), 143-150 (1983)
顯示於類別:	[嘉南學報] 9 期 (1983) [ 食品科技系 ] 期刊論文

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