High performance Liquid Chromatography of Alkaline-Soluble Food Additives

CNU IR > Chna Nan Annual Bulletin > Vol.9 (1983) > Item 310902800/25954

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Title:	High performance Liquid Chromatography of Alkaline-Soluble Food Additives
Authors:	Dong-Bor Yeh
Contributors:	食品衛生科
Date:	1983
Issue Date:	2012-11-16 09:01:25 (UTC+8)
Abstract:	A suitable procedure for the separation of several alkaline-soluble food additives, including saccharin, caffeine, sorbic acid, benzoic acid, para-hydroxy benzoic acid and salicylic acid is worked out. In this study, the food additives tested were completely separated and distinguished from Vit C within 20 minutes by high pressure liquid chromatography (Hitachi 638-.50) on a small reverse-phase column (LiChrosorb RP-18; 15x0.4 cm).An isocratic Na-acetate buffer containg 2-4 % 2-propanol was used as eluent, and the min. amount detectable was in the nanogram range. Furthermore, by referring the elution profiles obtained from a dual-wavelength UV monitor (Hitachi 635M) and connected daual-pen recorder (Hitachi 056), the identification of additive(s) in food is more reliable than that judged only by the retention time.
Relation:	嘉南學報 9, A52 轉載自 Program in the 21st Annual Meeting of Chinese Agricultural Chemical Society ,1983,28
Appears in Collections:	[Chna Nan Annual Bulletin] Vol.9 (1983) [Dept. of Food Science & Technology] Periodical Articles

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