| 摘要: | 本研究為探討北冬蟲夏草(蛹蟲草)體內與體外生物活性，分三年完成。第一年計畫為比較北冬蟲夏草水萃物(water extracts of Cordyceps militaris; CME)，冬蟲夏草水萃物(water extracts of Cordyceps sinensis; CSE)，市售冬蟲夏草水萃物(water extracts of commercial Cordyceps sinensis; CCSE)對細胞模式之護肝機能性表現。結果顯示CME、CSE與CCSE單獨處理對人類肝細胞株HepG2並無明顯毒性。CME，CSE與CCSE可以有效抑制tert-Butylhydroperoxide (t-BHP) 誘發細胞毒性，而CME與CSE二者的效果在統計上並無差異(P>0.05)。同時， CME，CSE與CCSE可以有效抑制t-BHP誘發細胞內氧化壓力的產生，且CSE之效用仍略高於CME，但統計上無差異(P>0.05)。另一方面，CME，CSE與CCSE也可以有效抑制t-BHP誘發細胞內Caspase 3 活性。以西方墨點分析法分析顯示CME，CSE與CCSE也可以有效抑制t-BHP誘發細胞內Caspase3蛋白之表現。綜合上述結果可知CME，CSE與CCSE對氧化壓力所引發之細胞凋亡具有氧化保護之機能作用，而其機轉可能與其降低胞內活性氧產生及抑制Caspase3活性有關。雖然， CME在本試驗模式效果略低於CSE，但是統計上並無差異(P>0.05)，顯示北冬蟲夏草水萃物對細胞模式具有顯著護肝機能性之表現。
In this three-year project, the major purpose is to investigate of the bioactivities of Cordyceps militaris in vivo and in vitro. The major goal of the first year is to compare hepatoprotective activities of CME, CSE, and CCSE, respectively, in cellular model. According to the data, CME, CSE, and CCSE did not exhibit obvious cytotoxicity toward HepG2 cells. On the other hand, the oxidative toxicity of t-BHP was decreased in the presence of CME, CSE, and CCSE respectively. No significant difference (p>0.05) was found between CME and CSE in protective effects of oxidative toxicity of t-BHP. Meanwhile, the intracellular oxidative stress induced by t-BHP in HepG2 cells was inhibited in the presence of CME, CSE, and CCSE, respectively. Further, the activity of intracellular caspase 3 induced by t-BHP was decreased in the presence of CME, CSE, and CCSE, respectively. By immunoblot, the protein levels of caspase 3 increased by t- BHP was inhibited in the presence of CME. According to the data mentioned above, the apoptosis progression induced by oxidative stress was inhibited by CSE, CME, and CCSE, respectively. The mechanism for the hepatoprotection in vitro may be attributed to decrease of oxidative stress and caspase 3 activity. This study indicated that CME displayed marked hepatoprotection in vitro. |
