人工培養的北冬蟲夏草子實體對mitomycin C 誘導基因毒性之保護作用

CNU IR > Chna Nan Annual Bulletin > No.37 (2011) > Item 310902800/25437

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Title:	人工培養的北冬蟲夏草子實體對mitomycin C 誘導基因毒性之保護作用 The protective effect of cultured fruiting body of Cordyceps militaris in mitomycin C-induced genotoxicity
Authors:	宋文君林恆弘施美份黃秀琴劉宗榮陳秋蘭
Contributors:	嘉南藥理科技大學藥物科技研究所嘉南藥理科技大學藥學系國立陽明大學環境衛生研究所
Keywords:	北冬蟲夏草基因毒性微核 Cordycepsmilitaris genotoxicity micronucleus
Date:	2011-12
Issue Date:	2012-06-08 14:21:20 (UTC+8)
Abstract:	中華冬蟲夏草〈中華蟲草〉是一種中國傳統滋補的昂貴中草藥材，因此市面上出現許多偽品，故人們開始尋找與中華蟲草具有相同活性的替代品。目前市面上常用北冬蟲夏草〈北蟲草〉作為中華蟲草的替代品。而中華蟲草的主要成分及相關的藥理活性已被許多科學家證實，但是有關北蟲草的研究卻不多，所以本研究之目的在評估人工培養的北蟲草子實體對mitomycin C 所誘導的基因毒性是否具有保護的作用。微核試驗是評估基因毒性的方法，以計數1000 個網狀細胞中出現多少具有微核的網狀細胞來加以評估，而mitomycin C〈MMC〉是一種具有基因毒性的抗腫瘤藥物。在此實驗中，我們先分別以胃管餵食ICR 小鼠125、250、500 或1000mg/kg 的人工培養北蟲草子實體懸浮液連續一週後，在第七天給予腹腔注射0.5 或1mg/kg 的MMC 以誘導基因毒性，並在腹腔注射MMC 後的第24、48及第72 小時分別進行眼窩週邊血採樣來計數微核的數量。本研究結果發現預先餵食1000mg/kg 人工培養北蟲草子實體的懸浮液連續十天後，對MMC 在第24 小時所誘導的基因毒性具有保護作用；而500mg/kg 人工培養北蟲草子實體的懸浮液則在MMC 注射後的第72 小時才有明顯減少的趨勢。綜合以上結果，本研究認為人工培養的北冬蟲夏草子實體對MMC 所誘導的基因毒性具有保護的作用。 Cordyceps sinensis (CS) is an expensive tonic Chinese herbal medicine. Therefore, people look for a similar pharmacological effects substitute of CS when many counterfeits and mimics of CS always found in markets, while Cordyceps militaris (CM) is a popularly using and cheaper natural of CS. The purpose of this study was evaluated the protective effect of cultured fruiting body of CM in mitomycin C-induced genotoxicity. The micronucleus test is an evaluated genotoxicity method by counting how many reticulocytes contained micronuclei (MN) in 1000 reticulocytes. And mitomycin C is a famous anti-tumor cytotoxic drug. In this study, we had designed to pre-treat ICR mice with 125, 250, 500 and 1000 mg/kg cultured fruiting body of CM (o.p.) for seven days, then given 0.5 mg/kg or 1 mg/kg MMC by intraperitoneal (i.p.) at the 7th day, then collected orbital peripheral blood of mice to count the number of MN after mitomycin C treatment at 24, 48 and 72 hours. We found that cultured fruiting body of CM decreased the frequency of MN after mitomycin C treatment at 24 hours (1000 mg/kg CM) and 72 hours (500, 1000 mg/kg) (p<0.05). In this study, our results suggest that cultured fruiting body of CM may contribute to the prevention of mitomycin C-induced genotoxicity.
Relation:	嘉南學報(科技類) 37期：p.26-31
Appears in Collections:	[Chna Nan Annual Bulletin] No.37 (2011) [Dept. of Pharmacy] Periodical Articles

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