炸油或藥物投與對維生素E代謝途徑之探討

CNU IR > Pharmacy and Science > Dept. of Health and Nutrition (including master's program) > MOST Project > Item 310902800/2542

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Title:	炸油或藥物投與對維生素E代謝途徑之探討 Vitamin-E Supplementation Restored Hepatic Vitamin-E Levels but not Protein Levels of Antioxidant Enzymes Decreased by Administration of Pregnenolone-16-Carbonitrile in Rats
Authors:	蕭慧美;HUEY-MEI SHAW
Contributors:	保健營養系
Keywords:	α-tocopherol TBARS antioxidant enzyme cytochrome P450 rat
Date:	2007
Issue Date:	2008-07-31 16:50:52 (UTC+8)
Publisher:	台南縣：嘉南藥理科技大學保健營養系
Abstract:	Pregnenolone-16-carbonitrile (PCN) is a synthetic steroid and an inducer of the cytochrome P450 3A gene in rats. The aim of this study was to evaluate the effects of PCN administration on vitamin-E status and antioxidant enzyme protein levels in rats fed a vitamin-E supplemented diet. Two groups of Wistar rats were fed for three weeks with a basal diet (containing 50 ppm of -tocopherol) or the same diet containing tenfold more -tocopherol. In the last three days, each group was divided into two subgroups which were given a single intraperitoneal injection of PCN at 75 mg/kg/day (P50 and P500 groups) or DMSO (C50 and C500 groups). PCN significantly reduced -tocopherol contents in the liver and plasma. Tenfold supplementation of -tocopherol (P500 group) could return liver -tocopherol levels to that of the C50 group. The TBARS concentration was significantly elevated by PCN administration in the liver and lung by Two-way ANOVA analysis. PCN showed a significant reduction in the protein levels of catalase and glutathione peroxidase (GPx). Dietary vitamin-E supplementation altered the protein levels of GPx and superoxide dismutase, but not catalase. Vitamin-E supplementation protected against PCN-induced lipid peroxidation and compromized vitamin-E status by CYP3A induction and antioxidant enzyme reduction. The mechanism of this protection appears to be due to scavenging the reactive metabolite by vitamin-E rather than increasing antioxidant enzyme levels.
Relation:	計畫編號：NSC96-2320-B-041-008
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] MOST Project

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