蛋白質缺乏誘導大鼠肝鐵堆積

CNU IR > Chna Nan Annual Bulletin > No.37 (2011) > Item 310902800/25428

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Title:	蛋白質缺乏誘導大鼠肝鐵堆積 Protein-depletion induces hepatic iron accumulation in rats
Authors:	黃惠玲蕭慧美蕭寧馨
Contributors:	嘉南藥理科技大學保健營養系台灣大學生化科技學系
Keywords:	蛋白質缺乏鐵調控蛋白(IRPs) 鐵蛋白大鼠 proteindepletion IRPs ferritin rats
Date:	2011-12
Issue Date:	2012-06-08 11:08:24 (UTC+8)
Abstract:	本研究目的在於探討膳食蛋白質缺乏對於大鼠鐵代謝的影響。採用六週大S.D. 雄鼠10 隻，分成兩組：正常控制組（N 組）與蛋白質缺乏組（0% protein，PF 組），每組各5 隻，飼養8 天後犧牲，取血液與肝臟分析鐵代謝相關指標。結果顯示，PF 組體重與血漿白蛋白顯著降低，血紅素與控制組相當，血球比容顯著高於C 組，肝鐵濃度、鐵蛋白（ferritin）量、粒線體aconitase (m-ACO)酵素量分別為C 組的1.8 倍、9 倍、1.7 倍，肝鐵顯著堆積，且鐵儲存指標（ferritin）與功能性指標（m-ACO）均顯著增加；肝臟鐵調控蛋白IRPs 之RNA 結合活性，IRP1 不受蛋白質缺乏的影響，IRP2 於PF 組顯著降低。結論：短期蛋白質缺乏，降低IRP2 活性，顯著增加肝鐵濃度與鐵蛋白量，導致肝鐵堆積。 The aim of this study was to investigate the effect of dietary protein depletion on the iron metabolism in rats. 10 male SD rats (6-wks-old) were assigned to 2 groups (n=5), normal control (N) and protein-free group (PF). After 8 d feeding, all animals were sacrificed by carbon dioxide asphyxiation. Blood and liver were collected for analysis of iron-associated parameters. Our results showed that PF group had significantly lower body weight and serum albumin compared with N group. Hb level had no difference between 2 groups, but Hct was higher in PF group. The hepatic iron, ferritin, and mACO in PF goup were 1.8, 9 and 1.7 fold, respectively. The IRP2 activity was significantly decreased in PF group. In summary, protein depletion showed a decrease in IRP2 activity and higher iron and ferritin contents in liver. There was significantly hepatic iron accumulation in protein-free group.
Relation:	嘉南學報(科技類) 37期：p.19-25
Appears in Collections:	[Chna Nan Annual Bulletin] No.37 (2011) [Dept. of Health and Nutrition (including master's program)] Periodical Articles

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