HPLC-fluorescence assay for measuring mosapride in small volumes of rat plasma

CNU IR > Pharmacy and Science > Dept. of Pharmacy > Periodical Articles > Item 310902800/25393

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/25393

Title:	HPLC-fluorescence assay for measuring mosapride in small volumes of rat plasma
Authors:	Ching-Ling Cheng Ya-Win Chang Chen-Hsi Chou
Contributors:	藥學系
Keywords:	mosapride gastroprokinetic agent HPLC-fluorescence pharmacokinetics rat
Date:	2010-03
Issue Date:	2012-06-05 10:10:32 (UTC+8)
Publisher:	Wiley
Abstract:	A simple and sensitive HPLC-fl uorescence assay was developed for the determination of a gastroprokinetic agent mosapride in small volumes of rat plasma. Samples (50 mL) were treated with 200 mL of the internal standard solution(cisapride, 0.1 mg/mL in acetonitrile). Chromatographic separation was achieved on a C18 column by gradient elution with the mobile phase of acetonitrile-water containing 20 mM potassium dihydrogen phosphate, at a fl ow rate of 1 mL/min. Fluorescence was measured with excitation and emission set at 315 and 354 nm, respectively. The retention time was about 16 min for cisapride and 20 min for mosapride. No endogenous ubstances were found to interfere. The calibration curve was linear from 0.015 to 10 mg/mL. The lower limit of quantifi cation was 0.015 mg/mL. The intra- and inter-day precision expressed as relative standard deviation did not exceed 7.7%, and the accuracy was within 4.7% deviation of the nominal concentration. The method was used successfully to investigate the disposition kinetics of mosapride in rats.
Relation:	Biomedical Chromatography 24(3) ：p.281-288
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles

Files in This Item:

File	Description	Size	Format
99_165_j.pdf		348Kb	Adobe PDF	292	View/Open
index.html		0Kb	HTML	2365	View/Open