Characterization of the Aspergillus nidulans monodictyphenone gene cluster

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Title:	Characterization of the Aspergillus nidulans monodictyphenone gene cluster
Authors:	Yi-Ming Chiang Edyta Szewczyk Ashley D. Davidson Ruth Entwistle Nancy P. Keller Clay C. C. Wang Berl R. Oakley
Contributors:	藥物科技研究所
Date:	2010-04
Issue Date:	2012-06-04 16:40:11 (UTC+8)
Publisher:	Amer Soc Microbiology
Abstract:	Deletion of cclA, a component of the COMPASS complex of Aspergillus nidulans, results in the production of monodictyphenone and emodin derivatives. Through a set of targeted deletions in a cclA deletion strain, we have identified the genes required for monodictyphenone and emodin analog biosynthesis. Identification of an intermediate, endocrocin, from an mdpHΔ strain suggests that mdpH might encode a decarboxylase. Furthermore, by replacing the promoter of mdpA (a putative aflJ homolog) and mdpE (a putative aflR homolog) with the inducible alcA promoter, we have confirmed that MdpA functions as a coactivator. We propose a biosynthetic pathway for monodictyphenone and emodin derivatives based on bioinformatic analysis and characterization of biosynthetic intermediates.
Relation:	Applied and Environmental Microbiology 76(7)：p.2067-2074
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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