Performance of oxygen-carbon-inducer releasing materials (OCIRM) on co-metabolize TCE and degradation of its metabolites were studied. Potential of the orange peel as the inducer was evaluated as compared to limonene solution. The experiment was conducted in batch mode to determine the optimum size of OCIRM and suitable carbon source. Results showed a 1.0 cm3 cube was the optimal size and sugarcane bagasse was a suitable C source in which the maximum organic releasing rate of 0.30 mg COD day-1 g-1 cube and dissolved oxygen concentration of 11.95 mg O2 L-1 were generated. Limonene in orange peel and in solution successfully induced monooxygenase enzyme to co-metabolize TCE. Optimal concentration ratio of contaminant to limonene was 1:2.5. OCIRM containing sugarcane bagasse and limonene in orange peel using a biofilm of aerobic sludge as the inoculum effectively co-metabolize TCE, cis-DCE and VC with maximum percentage removal of 56, 61 and 72%, respectively.
