RGNNV induces mitochondria-mediated cell death via newly synthesized protein dependent pathway in fish cells

doi:10.1016/j.fsi.2010.05.003

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 食品科技系 > 期刊論文 > Item 310902800/25229

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標題:	RGNNV induces mitochondria-mediated cell death via newly synthesized protein dependent pathway in fish cells
作者:	Horng-Cherng Wu Jen-Leih Wu Heuy-Ling Chu Yu-Chin Su Jiann-Ruey Hong
貢獻者:	食品科技系
關鍵字:	Nervous necrosis virus Necrotic cell death Mitochondria RNA viral genome RNA interference Viral death inducer
日期:	2010-09
上傳時間:	2012-05-22 13:53:30 (UTC+8)
摘要:	The RNA nervous necrosis virus induces necrotic cell death in fish; however, the molecular mechanism remains unknown. In this study, we demonstrated that beta-nodavirus-induced mitochondria-mediated dependent cell death is through newly synthesized protein dependent pathway in replication cycle. We determined that newly synthesized protein dependent pathway is required for red-spotted grouper nervous necrosis virus (RGNNV)-induced cell death. UV irradiation of the virus effectively blocked viral replication and cell death. Next, RGNNV RNA-dependent RNA polymerase (RdRp or protein A) was cloned and its involvement in RNA genome replication and viral protein synthesis was analyzed. Protein A was initially expressed 48 h post-infection and localized to the cytoplasm. Knockdown of protein A expression completely blocked viral genomic replication and expression of viral protein expression RNA1 small hairpin RNA (shRNA) producing cell lines, which coincided with inhibition of phosphatidylserine exposure, mitochondria-mediated death signaling, and increased cell viability 72 h post-infection. Furthermore, RGNNV-induced mitochondria-mediated caspase-3-independent necrotic cell death is dependent on viral synthesized protein dependent pathway at the middle-late replication stage. Taken together, for instance these results suggested that RGNNV induces cell death may require newly synthesized protein for triggering host mitochondria-mediated cell death. These findings may provide new insights into RNA viral pathogenesis.
關聯:	Fish & shellfish immunology 29(3)：p.451-463
顯示於類別:	[ 食品科技系 ] 期刊論文

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