Upregulation of lipogenesis and protein tyrosine phosphatase-1B expression in the liver of wistar rats with metabolic syndrome chronically induced by drinking sucrose water

doi:10.1159/000321966

CNU IR > Pharmacy and Science > Dept. of Health and Nutrition (including master's program) > Periodical Articles > Item 310902800/25226

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Title:	Upregulation of lipogenesis and protein tyrosine phosphatase-1B expression in the liver of wistar rats with metabolic syndrome chronically induced by drinking sucrose water
Authors:	Chun-Yin Huang Yu-Shun Lin Gou-Chun Chen Hui-Ling Huang Shu-Han Chuang Pei-Min Chao
Contributors:	保健營養系
Keywords:	Metabolic syndrome Sucrose-containing drinking water Sterol regulatory element-binding protein-1c Carbohydrate response element-binding protein Protein tyrosine phosphatase-1B
Date:	2010-11
Issue Date:	2012-05-21 16:30:04 (UTC+8)
Abstract:	Background: Establishing animal models with metabolic disorders similar to human metabolic syndrome (MS) is important. In terms of eliciting a full array of MS, we have previously shown that Wistar rats are more responsive to sucrose water drinking than are C57BL/6J mice. This study was aimed at investigating the underlying molecular mechanism of sucrose water-induced MS in Wistar rats. Methods: Male Wistar rats were divided into 2 groups (n = 8 for each group) which were given plain water (C group) or 30% sucrose water (SW group) to drink ad libitum. After 20 weeks, the transcriptional levels and protein translocation of hepatic sterol regulatory element-binding protein-1c (SREBP-1c) and carbohydrate response element-binding protein (ChREBP) as well as the protein levels of protein tyrosine phosphatase-1B (PTP-1B) in insulin-responsive tissues (liver, muscle, and adipose tissue) were measured. Results: The sucrose water regimen successfully elicited visceral obesity, hypertriglyceridemia, insulin resistance, and high blood pressure. The upregulation of de novo lipogenesis in the liver of the sucrose water-treated rats was demonstrated by an increased activity of enzymes, mRNA levels of lipogenic proteins, and nuclear levels of SREBP-1c and ChREBP. Moreover, in the sucrose water-treated rats, protein levels of PTP-1B were significantly increased in liver and skeletal muscle but decreased in adipose tissue. Conclusion: The susceptibility of Wistar rats to sucrose water-induced MS is associated with the transactivation of SREBP-1c and ChREBP in the liver, and PTP-1B is involved in the upregulation of de novo lipogenesis in the liver and the pathology of systemic insulin resistance in rats with MS chronically induced by drinking sucrose water. Copyright [copy 2010 S. Karger AG, Basel
Relation:	Annals of Nutrition and Metabolism, v.57n.3-4, pp.169-176
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles

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