α-Solanine inhibits human melanoma cell migration and invasion by reducing matrix metalloproteinase-2/9 activities

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標題:	α-Solanine inhibits human melanoma cell migration and invasion by reducing matrix metalloproteinase-2/9 activities
作者:	Yuan-Wei Shih Tzu-Tsung Chang Chien Li-Heng Fang Hsiang-Ching Huang Pin-Shern Chen
貢獻者:	生物科技系
關鍵字:	α-solanine migration invasion matrix metalloproteinase
日期:	2010-10
上傳時間:	2012-03-30 15:17:54 (UTC+8)
摘要:	α-Solanine, a naturally occurring steroidal glycoalkaloid in potato sprouts, was found to possess anti-carcinogenic properties, such as inhibiting proliferation and inducing apoptosis of tumor cells. However, the effect of α-solanine on cancer metastasis remains unclear. In the present study, we examined the effect of α-solanine on metastasis in vitro. Data demonstrated that α-solanine inhibited proliferation of human melanoma cell line A2058 in a dose-dependent manner. When treated with non-toxic doses of α-solanine, cell migration and invasion were markedly suppressed. Furthermore, α-solanine reduced the activity of matrix metalloproteinase-2 (MMP-2) and MMP-9, which are involved in the migration and invasion of cancer cells. Our biochemical assays indicated that α-solanine potently suppressed the phosphorylation of c-Jun N-terminal kinase (JNK), phosphatidylinositide-3 kinase (PI3K) and Akt, while it did not affect phosphorylation of extracellular signal regulating kinase (ERK). In addition, α-solanine significantly decreased the nuclear level of nuclear factor kappa B (NF-κB), suggesting that α-solanine inhibited NF-κB activity. Taken together, the results suggested that α-solanine inhibited migration and invasion of A2058 cells by reducing MMP-2/9 activities. It also inhibited JNK and PI3K/Akt signaling pathways as well as NF-κB activity. These findings reveal new therapeutic potential for α-solanine in anti-metastatic therapy.
關聯:	Biological & Pharmaceutical Bulletin 33(10)：p.1685-1691
显示于类别:	[生物科技系(所)] 期刊論文

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