α-Chaconine inhibits angiogenesis in vitro by reducing matrix metalloproteinase-2

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/25162

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/25162

Title:	α-Chaconine inhibits angiogenesis in vitro by reducing matrix metalloproteinase-2
Authors:	Ming-Kun LU Pei-Hsieng CHEN Yuan-Wei SHIH Ya-Ting CHANG En-Tze HUANG Cheng-Ruei LIU Pin-Shern CHEN
Contributors:	生物科技系
Keywords:	α-chaconine angiogenesis matrix metalloproteinase-2 c-Jun-N-terminal kinase Akt neuclear factor kappa B
Date:	2010-04
Issue Date:	2012-03-30 15:17:53 (UTC+8)
Abstract:	α-Chaconine, a naturally occurring steroidal glycoalkaloid in potato sprouts, was found to possess anti-carcinogenic properties, such as inhibiting proliferation, migration, invasion, and inducing apoptosis of tumor cells. However, the effect of α-chaconine on tumor angiogenesis remains unclear. In the present study, we examined the effect of α-chaconine on angiogenesis in vitro. Data demonstrated that α-chaconine inhibited proliferation of bovine aortic endothelial cells (BAECs) in a dose-dependent manner. When treated with non-toxic doses of α-chaconine, cell migration, invasion and tube formation were markedly suppressed. Furthermore, α-chaconine reduced the expression and activity of matrix metalloproteinase-2 (MMP-2), which is involved in angiogenesis. Our biochemical assays indicated that α-chaconine potently suppressed the phosphorylation of c-Jun N-terminal kinase (JNK), phosphatidylinositide-3 kinase (PI3K) and Akt, while it did not affect phosphorylation of extracellular signal regulating kinase (ERK) and p38. In addition, α-chaconine significantly increased the cytoplasmic level of inhibitors of κBα (IκBα) and decreased the nuclear level of nuclear factor kappa B (NF-κB), suggesting that α-chaconine could inhibit NF-κB activity. Furthermore, the treatment of inhibitors specific for JNK (SP600125), PI3K (LY294002) or NF-κB (pyrrolidine dithiocarbamate) to BAECs reduced tube formation. Taken together, the results suggested that α-chaconine inhibited migration, invasion and tube formation of BAECs by reducing MMP-2 activities, as well as JNK and PI3K/Akt signaling pathways and inhibition of NF-κB activity. These findings reveal a new therapeutic potential for α-chaconine on anti-angiogenic therapy.
Relation:	Biol. Pharm. Bull.33(4)：p.622-630
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

Files in This Item:

File	Description	Size	Format
99_32_j.pdf		3503Kb	Adobe PDF	305	View/Open
index.html		0Kb	HTML	2384	View/Open

Loading...