A Recombinant Metalloprotease Antigen of Vibrio vulnificus Elicits Protective Antibodies in a Murine Model

doi:10.1111/j.1472-765X.2009.02771.x

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/25154

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Title:	A Recombinant Metalloprotease Antigen of Vibrio vulnificus Elicits Protective Antibodies in a Murine Model
Authors:	Y.-C. Chen C.-C. Chang S.-Y. Chang J.-H. Su
Contributors:	生物科技系
Keywords:	infection metalloprotease protective effect vaccine Vibrio vulnificus
Date:	2010-02
Issue Date:	2012-03-30 15:17:44 (UTC+8)
Publisher:	Wiley-Blackwell
Abstract:	Aims: To investigate whether Vibrio vulnificus metalloprotease (VvpE) can induce the production of specific anti-VvpE antibody to confer effective protection against Vibrio vulnificus infection and to evaluate the possibility of VvpE as a potential vaccine candidate against disease caused by V. vulnificus. Methods and Results: The gene encoding the 65-kDa VvpE of V. vulnificus was amplified by PCR and cloned into the expression vector pET21(b). The recombinant VvpE of V. vulnificus was expressed in Escherichia coli BL21(DE3). This His6-tagged VvpE was purified and injected intramuscularly into mice to evaluate its ability to stimulate immune response. Specific antibody levels were measured by ELISA. The 75% protective efficacy of recombinant VvpE was evaluated by active immunization and intraperitoneal challenge with V. vulnificus in mice. Conclusions: The recombinant His6-tagged VvpE of V. vulnificus is capable of inducing high antibody response in mice to confer effective protection against lethal challenge with V. vulnificus. VvpE might be a potential vaccine candidate to against V. vulnificus infection. Significance and Impact of the Study: This study uses His6-tagged VvpE to act as vaccine that successfully induces effective and specific anti-VvpE antibody and offers an option for the potential vaccine candidate against V. vulnificus infection.
Relation:	Letters in Applied Microbiology 50(2)：p.168-172
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles [Dept. of Food Science & Technology] Periodical Articles

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