Molecular Cloning mzd Structural Analysis of Murine Thymidine Kinase Genomic and cDNA Sequences

Chia Nan University of Pharmacy & Science Institutional Repository > 嘉南學報 > 12期 (1986) > Item 310902800/25057

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標題:	Molecular Cloning mzd Structural Analysis of Murine Thymidine Kinase Genomic and cDNA Sequences
作者:	PIN-FANG LIN HOWARD B. LIEBERMAN DONG-BOR YEH TIAN XU SHOU-YUAN ZHAO FRANK H RUDDLE
貢獻者:	食品衛生科
日期:	1986
上傳時間:	2012-02-24 16:04:41 (UTC+8)
摘要:	Two functional cytosolic thymidine kinase (tk) cDNA clones were isolated from a mouse L-cell library An RNA blot analysis indicated that one of these clones contains a nearly full-length tk sequence and that LTK￣cells contain little or no TK message. The nucleotide sequences of both clones were determined, and the functional mouse tk cDNA contains 1,156 base pairs. An analysis of the sequence implied that there is an untranslated 32-nucleotide region at the 5 end of the mRNA, followed by an open reading frame of 699 nucleotides. The 3 untranslated region is 422 nucleotides lonpg. Thus , the gene codes for a protein containing 233 amino acids, with a molecular weight of 25,873. A comparison of the coding sequences of the mouse tk cDNA with the human and chicken tk genes revealed about 86 and 70% homology, respectively. We also isolated the tk gene from a mouse C57BL/10J cosmid library. The structural organization was determined by a mouse tk cDNA. The tk gene spans approximately 11 kilobases and contains at least five introns. Southern blot analysis revealed that this gene is deleted in mouse LTK￣cells, consistent with the inability of these cells to synthesize TK message. This analysis also shovved that tk-related sequences are present in the genomes of several mouse strains, as well as in LTK-cells. These segments may represent pseudogenes.
關聯:	嘉南學報 12：p.A69-A76 轉載 Molecular and Cellular Biology p.3149-3156 (Nov. 1985)
显示于类别:	[嘉南學報] 12期 (1986) [ 食品科技系 ] 期刊論文

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