E-Learning is thought of as an innovative approach to enhance nurses’ care service knowledge. xtensive research has provided rich information toward system development, courses design and nurses’ satisfaction with an e-Learning system. However, a comprehensive view in understanding nursing e-Learning system success is an important but less focused-on topic. The purpose of this research is to explore nurses’ e-Learning system use intention and satisfaction relating to net benefits based on the modified DeLone and McLean’s Information System Success Model (ISSM). The study used a structured, self-administered questionnaire and collected 208 valid nurses’ responses from 21 of Taiwan’s mid-and-large-scale hospitals (i.e. medical centers and regional hospitals) that have implemented nursing e-Learning systems. The result, after structural equation modeling (SEM) analysis, confirms that the ISSM is sufficient to explore the nurses’ e-Learning use in terms of “Intention to Use,” “User Satisfaction” and “Net Benefits.” However, while the three exogenous quality factors (System Quality, Information Quality and Service Quality) were all found to be critical factors affecting “User Satisfaction,” only “Information Quality” showed a direct impact on the “Intention to Use” the e-Learning system. This study provides useful insights for evaluating nursing e-Learning qualities as well as an understanding of nurses’ intentions and satisfaction related to performance benefits.