以修飾矽凝膠結合蛋白質線上分解之微晶片DNA 純化裝置

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > MOST Project > Item 310902800/2471

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Title:	以修飾矽凝膠結合蛋白質線上分解之微晶片DNA 純化裝置
Authors:	王貞雅
Contributors:	生物科技系
Keywords:	核酸純化矽磁微粒四甲基矽氧烷四乙基矽氧烷 3-氨基丙基三甲基矽氧烷胃蛋白酶 silica magnetite plasmid DNA extraction purification
Date:	2006
Issue Date:	2008-07-31 16:50:08 (UTC+8)
Publisher:	台南縣：嘉南藥理科技大學生物科技系
Abstract:	本計畫利用溶膠凝膠法於順磁性奈米微粒表面包覆不同矽氧烷基聚合物，如四甲基矽氧烷(tetramethoxysilane；TMOS )、四乙基矽氧烷(tetraethoxysilane；TEOS )、3-氨基丙基三甲基矽氧烷(3-aminopropyltriethoxysilane；APTES)，探討這些不同修飾的矽磁微粒對DNA 的吸附特性，其中以四甲基矽氧烷修飾矽磁微粒吸附DNA 的能力最好；在pH 2.8 - pH 7.8 溶液中，矽磁微粒吸附DNA 的能力隨酸鹼值下降而提高。所製備之矽磁微粒已成功於真實樣品，如菌液中，吸附DNA 並進行特定基因之檢測。 The extraction and isolation of DNA from biological samples is one of the most commonly used procedures in genetics, molecular biology. The quality of isolated nucleic acids is critical in obtaining accurate and meaningful analytical data. The extraction of DNA from crude biological matrixes is commonly performed by solid-phase extraction (SPE), which has largely replaced liquid-liquid extraction for purifying DNA. The most widely used solid supports for SPE of DNA have been silica-based particles. Silica particles modified to create a cationic surface have also been shown to bind plasmid DNA electrostatically, and thus could be used as a solid-phase matrix for DNA purification. Though numerous methods that prepare magnetite/silica nanocomposites for adsorbing DNA have been developed for purifying DNA, the search for the best purification procedure and comparison of different materials in terms of the extraction efficiency of DNA has gained less attention. Our research was focused on testing different magnetite/silica nanocomposites based on covalent or noncovalent modification of the silica surface with 3-aminopropyltriethoxysilane (APTES) in search of the best efficiency, fewer processing steps, and shorter analysis times for DNA extraction.
Relation:	計畫編號：NSC 95-2113-M-041-004
Appears in Collections:	[Dept. of Biotechnology (including master's program)] MOST Project

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