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    標題: 以修飾矽凝膠結合蛋白質線上分解之微晶片DNA 純化裝置
    作者: 王貞雅
    貢獻者: 生物科技系
    關鍵字: 核酸純化
    矽磁微粒
    四甲基矽氧烷
    四乙基矽氧烷
    3-氨基丙基三甲基矽氧烷
    胃蛋白酶
    silica
    magnetite
    plasmid
    DNA
    extraction
    purification
    日期: 2006
    上傳時間: 2008-07-31 16:50:08 (UTC+8)
    出版者: 台南縣:嘉南藥理科技大學生物科技系
    摘要: 本計畫利用溶膠凝膠法於順磁性奈米微粒表面包覆不同矽氧烷基聚合物,如四甲基矽氧
    烷(tetramethoxysilane;TMOS )、四乙基矽氧烷(tetraethoxysilane;TEOS )、3-氨基丙基三甲
    基矽氧烷(3-aminopropyltriethoxysilane;APTES),探討這些不同修飾的矽磁微粒對DNA 的
    吸附特性,其中以四甲基矽氧烷修飾矽磁微粒吸附DNA 的能力最好;在pH 2.8 - pH 7.8 溶
    液中,矽磁微粒吸附DNA 的能力隨酸鹼值下降而提高。所製備之矽磁微粒已成功於真實樣
    品,如菌液中,吸附DNA 並進行特定基因之檢測。
    The extraction and isolation of DNA from biological samples is one of the most commonly used
    procedures in genetics, molecular biology. The quality of isolated nucleic acids is critical in
    obtaining accurate and meaningful analytical data.
    The extraction of DNA from crude biological matrixes is commonly performed by solid-phase
    extraction (SPE), which has largely replaced liquid-liquid extraction for purifying DNA. The most
    widely used solid supports for SPE of DNA have been silica-based particles. Silica particles
    modified to create a cationic surface have also been shown to bind plasmid DNA electrostatically,
    and thus could be used as a solid-phase matrix for DNA purification. Though numerous methods
    that prepare magnetite/silica nanocomposites for adsorbing DNA have been developed for purifying
    DNA, the search for the best purification procedure and comparison of different materials in terms
    of the extraction efficiency of DNA has gained less attention. Our research was focused on testing
    different magnetite/silica nanocomposites based on covalent or noncovalent modification of the
    silica surface with 3-aminopropyltriethoxysilane (APTES) in search of the best efficiency, fewer
    processing steps, and shorter analysis times for DNA extraction.
    關聯: 計畫編號:NSC 95-2113-M-041-004
    顯示於類別:[生物科技系(所)] 科技部計畫

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