埃及斑蚊幼蟲抗藥性與解毒酵素相關性分析

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標題:	埃及斑蚊幼蟲抗藥性與解毒酵素相關性分析 Correlation Analysis of the Insecticide Resistance and Detoxification Enzymes in the Aedes aegypti (L.) Larvae
作者:	姜寬盈
貢獻者:	嘉南藥理科技大學：生物科技系暨研究所羅怡珮
關鍵字:	埃及斑蚊百滅寧抗藥性解毒酵素誘導試驗 Aedes aegypti Permethrin Induction of the detoxification enzyme Insecticide resistance
日期:	2009
上傳時間:	2011-10-27 14:43:01 (UTC+8)
摘要:	以亞培松（Temephos）、陶斯松（Chlorpyrifos）、撲滅松（Fenitrothion）、亞特松（Pirimifosmethyl）等四種有機磷殺蟲劑，及必列寧（Pyrethrins）、百滅寧（Permethrin）、芬化利（Fenvalerate）、賽滅寧（Cypermethrin）四種合成除蟲菊類殺蟲劑進行埃及斑蚊幼蟲之生物檢測。測試各供試藥劑對對敏感品系、台南地區及高雄地區埃及斑蚊的半數致死濃度(LC50)，並計算抗性比值。結果顯示，台南地區及高雄地區埃及斑蚊幼蟲對有機磷殺蟲劑尚未產生抗藥性的情形，有機磷殺蟲劑對野外的埃及斑蚊幼蟲仍具有撲殺的效果。除關廟品系外，台南地區及高雄地區埃及斑蚊幼蟲對四種除蟲菊類殺蟲劑之抗性比值皆大於10，顯示台南地區及高雄地區的埃及斑蚊已對除蟲菊類殺蟲劑包括必列寧、百滅寧、賽滅寧及芬化利產生抗藥性。在多濃度安丹抑制實驗中，97台南東區品系與高雄苓雅品系的乙醯膽鹼酯酶抑制曲線的變化，都與敏感品系相同，因此推論台灣地區埃及斑蚊的乙醯膽鹼酯酶並未產生”質”的突變，此結果與生物檢測結果相符。在解毒酵素分析結果，高雄地區埃及斑蚊四齡幼蟲在單氧酶、酯酶及麩胺基硫轉移酶等三類解毒酵素的表現量都較敏感品系高，但是台南地區埃及斑蚊四齡幼蟲則與高雄地區品系有不同的結果，可能兩個地區對殺蟲劑的抗藥機制並不相同。以百滅寧的亞致死劑量進行埃及斑蚊幼蟲解毒酵素誘導試驗，在台南市東區埃及斑蚊幼蟲的解毒酵素活性增加情形與接觸殺蟲劑的時間長短有關，並且與敏感品系間具有顯著性的差異。但是其他品系的實驗結果，缺乏一致性，短時間暴露殺蟲劑對不同地區品系埃及斑蚊幼蟲的影響並不一致，是否與各地區用藥習慣的不同或各地區抗藥機制差異有關，尚待進一步釐清及探討。 Field collected Aedes aegypti from each part of Tainan and Kaohsiung districts were subjected to bioassay for their susceptibilities to Temephos, Chlorpyrifos, Fenitrothion, Pirimifosmethyl, Pyrethrins, Permethrin, Fenvalerate, Cypermethrin and compared with two susceptible laboratory strains (NS strain and Bora Bora strain) by using dipping method. The resistance ratio between the 50% lethal concentration value (RR) of the field strains and the NS strain shows that the field strain were widespread, significant resistance to pyrethroid insecticides but not resistance to organophosphorus insecticides. In TPP test (insecticide-insensitive acetylcholinesterase test), the AChE residual activities of field strain compared with susceptible strain were the same in the presence of increasing concentration of propoxur. The TPP test indicate that the point mutation of acetylcholinesterase of Aedes aegypti was not found in the southern of Taiwan. Microplate assays were performed to measure levels of α-esterase, β-esterase, glutathione-S-transferase, monooxygenase enzymes. The patterns of elevate levels of detoxification enzymes in Kaohsiung districts and the Tainan districts strains were different. Comparison between bioassays and biochemical assays, the higher levels glutathione-S-transferase activity was significant correlation with the 50% lethal concentration value of pyrethroid insecticides. The effect of induction detoxification enzymes activity by exposure of Aedes aegypti larvae to sub-lethal dose of permethrin was investigated. The induction detoxification enzymes activity by permethrin in the 97 Tainan East district strain were significant higher than NS strain. The induction effect was no consistency between the experimental field strains in the 1 hr, sub-lethal dose exposure treatment. The mechanisms of insecticide resistance need to be clarified and explored further.
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