| 摘要: | 本研究目的為利用超臨界二氧化碳流體萃取及分餾方法提昇薑根莖中6-gingerol含量。
以0.297~0.840 mm，0.840~2.000 mm及2.000 mm以上三種不同大小薑顆粒，35、40及50 ℃三種不同溫度，150、200及250 bar三種不同壓力作為超臨界二氧化碳流體萃取條件之三種主要變數因素，採取三因素三水平正交試驗法，進行九個薑樣品之超臨界二氧化碳流體萃取，並以HPLC分析所得萃取物中6-gingerol含量，結果顯示以0.840~2.000 mm之薑顆粒於35 ℃及200 bar條件下萃取所得薑油樹脂之6-gingerol含量最高（20.23%）。
為了再提昇薑油樹脂中6-gingerol之含量，依上條件進行280 kg大量超臨界二氧化碳流體萃取，所得薑油樹脂再進行超臨界二氧化碳流體分餾萃取，以35、45及55 ℃三種不同溫度，80、100及120 bar三種不同壓力，超臨界二氧化碳流體與薑油樹脂質量以5:1、10:1及15:1三種不同流率比及分離槽收集液回流抽取次數為0、1及2次等作為分餾萃取條件之四個主要變數，採取四因素三水平正交試驗法進行十個薑油樹脂樣品之超臨界二氧化碳流體分餾萃取，並以HPLC分析所得分餾萃取物之6-gingerol含量，結果顯示以120 bar、35 ℃、超臨界二氧化碳流體與薑油樹脂質量流率比為10:1及分離槽收集液回流抽提2次的條件下萃取所得萃取物之6-gingerol含量最高為68.83 %。
本研究可利用超臨界二氧化碳流體萃取及分餾方法提升薑中6-gingerol含量，未來可應用於產業界量產。
The purpose of this study is to increase the content of 6-gingerol extracted from ginger rhizome (ZO) by means of two methods: Supercritical Carbon Dioxide Fluid Extraction (SCO2FE) and Supercritical Carbon Dioxide Fluid Fractionation Extraction (SCO2FFE).
Three major variables were taken into account in the experiment of SCO2FE: ZO particle sizes (0.297-0.840 mm, 0.840-2.000 mm and above 2.000 mm), temperatures (35, 40, and 50 degree Celsius), and pressures (150, 200, and 259 bar). In consideration of three major variables, Three Factors at Three Level Orthogonal experimental method was applied in this study to examine 9 ZO samples. Meanwhile, analysis of High-Performance Liquid Chromatography (HPLC) was conducted to determine the content of 6-gingerol. As a result, particle size of 0.840-2.000 mm ZO was believed to extract the most optimized level 6-gingerol (20.23%) under the conditions of temperature at 35 degree Celsius and pressure at 200 bar.
Sequentially, to further increase the contents of 6-gingerol extracted from ZOE, applying the ZOE obtained from previous phase, four major variables are accounted for in this experiment of SCO2FFE : temperatures (35, 40, and 50 degree Celsius), pressures (150, 200, and 259 bar), flow-rate ratio of supercritical Carbon Dioxide fluid and ZOE (5:1, 10:1, 15:1), and recycle frequency of collected solution from separator (0. 1, 2). Adapting Four Factors at Three Level Orthogonal experimental method, ten ZOE samples were examined by means of SCO2FFE. Combined with results generated from HPLC method, it indicates the most optimized results (68.83%) obtained under the conditions of temperature at 35 degree Celsius, pressure at 120 bars, flow-rate ratio at 10:1, and recycle frequency at 2.
This research aims to advise correlated industries to further the application of SCO2FE and SCO2FFE in exaction to optimize the content of 6-gingerol. |
