乳酸菌抑制幽門桿菌及其尿素酶活性之探討

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標題:	乳酸菌抑制幽門桿菌及其尿素酶活性之探討 Inhibition of Helicobacter pylori and associated urease by lactic acid bacteria
作者:	陳琮漢
貢獻者:	嘉南藥理科技大學：生物科技系暨研究所王淑珍張竣凱
關鍵字:	幽門螺旋桿菌乳酸菌細菌素尿素酶活性 Lactic acid bacteria (LAB) Helicobacter pylori bacteriocin urease activity
日期:	2011
上傳時間:	2011-10-25 15:30:04 (UTC+8)
摘要:	幽門桿菌感染是全世界常見的疾病之一，平均感染率高達50％以上，許多胃部疾病與幽門桿菌有關係，如胃癌中的胃腺癌、胃黏膜淋巴組織的淋巴瘤皆與幽門桿菌感染有關。最近，益生菌乳酸菌對人體有益的功能，如對腸道有害菌的抑制，抗癌及免疫的效果，降低膽固醇及改善營養等，與抑制幽門桿菌的機制在近年來也不斷被證實。因此，本篇將針對乳酸菌抑制幽門桿菌的機制及其能力進行探討。本研究以洋菜擴散法與生長抑制試驗進行分析，將分離之乳酸菌株發酵產物對2株幽門桿菌BCRC 17023、BCRC 15415進行生長抑制作用，共篩選出15株乳酸菌對幽門桿菌具有抑制作用，且抑制之效果優於 L. lactis subsp. lactis BCRC10791乳酸菌株。在體外試驗中，12.5% 乳酸菌發酵液為抑制幽門桿菌之最低抑制濃度，且實驗結果顯示，有10株乳酸菌株抑制幽門桿菌效果明顯優於BCRC 10791與 B. lactis BCRC 17394 (Bb-12)等乳酸菌株，其抑制幽門桿菌生長之效果可達到70％以上；此外，由於幽門桿菌內部及表面都具有很高的尿素酶活性，故將10株乳酸菌發酵液進行抑制幽門桿菌尿素酶活性探討，結果顯示，B0001、B0003、B0055、B0077、B0155等乳酸菌株對幽門桿菌BCRC 17023具有較佳抑制能力；B0055、B0056等乳酸菌株對幽門桿菌BCRC 15415具有較佳的抑制能力。由上述篩選出的乳酸菌株進行16S rDNA序列分析，結果顯示，乳酸菌B0001、B0003、B0055、B0077、B0155為 Lactobacillus plantarum，乳酸菌B0056為 Pediococcus pentosaceus。在細胞吸附試驗中發現乳酸菌發酵液能有效降低幽門桿菌吸附Caco-2與TSGH9201之菌落數。綜合上述研究結果顯示，本實驗篩選出的6株乳酸菌菌株所產生之細菌素均具有抑制幽門桿菌生長與其尿素酶活性的能力及降低幽門桿菌吸附細胞之能力。 Helicobacter pylori that infects over 50% of the population in world wide, has been linked to the development of gastric adeno-carcinoma and mucosa-associated lymphoid tissue lymphoma. Recently, lactic acid bacteria (LAB) has attracted much attention due to their potential inhibition of pathogen including H. pylori , Streptococcus mutans and Staphyloccus aureus. Therefore, the effects of lactic acid bacteria isolated from fermented vegetables on H.pylori were tested. A standard antimicrobial plate well diffusion assay was employed to examine inhibitory effects on H. pylori BCRC17023 and BCRC15415. 15 strains of LAB demonstrated inhibition of Helicobacter pylori growth in comparison with L. lactis subsp. lactis BCRC10791. In vitro test, the concentration of spend culture supernatant (SCS) against BCRC17023 and BCRC15415 was served in 12.5%. Among of them, 10 strains of LAB showed a significant inhibition rate (70%) against H. pylori compared to BCRC10791 and B. lactis BCRC17394 (Bb-12). Further, the effect of LAB strains on the urease activity of H.pylori was examined. The urease activity of H. pylori BCRC17023 was significantly inhibited by the presence of B0001, B0003, B0055, B0077 and B0155-SCS. H. pylori BCRC15415 was significantly inhibited by B0055, B0056-SCS. Strains of B0001, B0003, B0055, B0077, B0155 were identified as Lactobacillus plantarum and B0056 was Pediococcus pentosaceus , based on 16S rDNA sequence. Treatment of H. pylori BCRC17023 and BCRC15415 with LAB-SCS significantly reduced the number of H. pylori adhering to the monolayer of Caco-2 and TSGH9201 cell lines.
關聯:	校內一年後公開，校外永不公開，學年度：99，100頁
顯示於類別:	[生物科技系(所)] 博碩士論文

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