本研究探討絡石藤（Trachelospermum jasminoides）乙醇萃取物之抗氧化與抑制酪胺酸酶效能。絡石藤乙醇萃取物經由乙酸乙酯及水分配層析後，分別使用silica gel 60和Diaion HP 20進行管柱層析，共分得13個區段（EA1-EA6和W1-W7），之後比較各區段之抗氧化與抑制酪胺酸酶效能。抗氧化試驗包括DPPH自由基清除效能、ABTS自由基清除能力、還原能力、抑制脂質過氧化能力。
實驗結果顯示，DPPH自由基清除效能、ABTS自由基清除能能力和還原能力，以水層粗萃物分離後的第3區段（W3）效果最好，W3在200 ppm時，DPPH自由基清除效能和ABTS自由基清除能能力之抑制率分別為91.9 %和90.1 %，其還原能力相當於34 ppm的維生素C，總酚含量測定發現每克W3萃取物含有相當於158.8 mg之gallic acid，而總類黃酮含量測定為每克萃取物含有相當於99.8 mg之rutin。在抑制脂質過氧化能力方面，則是乙酸乙酯層粗萃物分離後的第2區段（EA2）最好，200 ppm時，抑制率達90.1 %。此外，抑制酪胺酸酶活性試驗顯示，於1000 ppm時，EA2抑制率可達86.8 %。綜合上述，粗萃物經過層析處理後，其區段粗萃物之抗氧化和抑制酪胺酸酶能力增加，應可為抗氧化和美白的潛力材料。 The antioxidant and antityrosinase activities of crude ethanolic extract and its fractions isolated by liquid-liquid extraction using ethyl acetate and water of Transchelospermum jasminoides were determined. The antioxidant activities were carried out using different in vitro assays including the inhibition of DPPH and ABTS radials, reducing power, and the inhibition of lipid peroxidation. Among these extracts, the ethyl acetate layer displayed the highest antioxidant and antityrosinase activities. In addition, these extracts were further fractionated by silica gel 60 and Diaion HP 20 columns into thirteen subfractions (EA1-EA6 and W1-W7).
The third fraction of water (W3) is the most efficient in DPPH and ABTS inhibition assay. At 200 ppm, the scavenging activity in DPPH assay is 91.9% and ABTS is 90.1%. The reducing power of W3 at 200 ppm is equal to that of 34 ppm vitamin C. The total phenol content of W3 is equal to 158.8 mg gallic acid and the total flavonoid content is equal to 99.8 mg rutin. The most active sample in inhibiting lipid peroxidation and tyrosinase is the second fraction of ethyl acetate (EA2). At 200 ppm, the inhibitory effect on lipid peroxidation is 90.1%. At 1000 ppm, the inhibitory effect on tyrosinase is 86.8%. In conclusion, the antioxidant and antityrosinase activities in the extracts of Trachelospermum jasminoides can play an important role in antioxidation and whitening products.
