| 摘要: | 本研究探討蝦紅素（astaxanthin）、香茹（Glossogyne tenuifolia）及苦茶（Camellia oleifera）花萃取物對3T3小鼠纖維母細胞的毒性程度，並且分析對於胞外之基質金屬蛋白酶（matrix metalloproteinases, MMPs）的活性抑制，再進一步探討對膠原蛋白是否具有保護作用，此外探討其抗氧化、美白、與抗菌活性。
實驗結果顯示，150 μg/ml蝦紅素水溶液可抑制MMP-2及MMP-9活性，減少 89.08% 的膠原蛋白降解，對於DPPH清除率高達97.95 ± 1.45%；10% 香茹萃取液與 2% 苦茶花萃取液對MMP-2及MMP-9皆具有非常明顯的抑制效果，並可完全達到保護膠原蛋白的作用，且DPPH清除率均達 90% 以上。美白效果的評估上，三者皆無明顯抑制酪胺酸酶活性的能力；抗菌活性方面，蝦紅素與香茹萃取液對於金黃色葡萄球菌、大腸桿菌及綠膿桿菌皆無明顯抑菌圈出現，苦茶花萃取液之原液對於金黃色葡萄球菌之抑菌圈為12.3 ± 0.5 mm，具中度抗菌活性，其餘亦無抑菌圈出現。
儘管以上物質在美白及抗菌活性方面沒有顯著的效果，然而三者不但皆具備強大的抗氧化活性，本研究亦證實它們具有抑制基質金屬蛋白酶的能力，並能夠減緩膠原蛋白的降解以達到抗老化，可應用於化粧品之有效成分。
In this study, cytotoxic levels of astaxanthin, Glossogyne tenuifolia and Camellia oleifera flowers on 3T3 mouse fibroblasts were measured by MTT assay. Further, we investigated the inhibitory effects on activation of secreted matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in culture medium using gelatin zymography. Collagen protection effect was researched subsequently using collagen assay kits. DPPH radicals scavenging activity, tyrosinase inhibition, agar disc diffusion method were performed for analyzing their antioxidant, whitening and antibacterial activity as well.
we observed that 150 μg/ml of astaxanthin aqueous solution which inhibited MMP-2, MMP-9 activities and reduced 89.08% of collagen decrement, was found 97.95±1.45% of high DPPH radicals scavenging activity. Both 10% of G. tenuifolia and 2% of C. oleifera flowers extract solution exhibited significant inhibitory effects on MMP-2, MMP-9 activities as well as complete protection effects for collagen. Moreover, they both manifested over 90% scavenging activity of DPPH radicals. In whitening and antibacterial experiment, these three subjects could not inhibit tyrosinase at all. Besides, astaxanthin aqueous solution and G. tenuifolia extract solution did not display obvious inhibition zones on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The extract solution of C. oleifera flowers appeared 12.3±0.5 mm of diameter of inhibition zones which is considered moderate activity on Staphylococcus aureus, but not shown antibacterial ability on the other two microorganisms.
Although these three subjects did not reveal powerful detections on whitening and antibacterial effects, they all possess strong antioxidant activities. Our results also demonstrate that astaxanthin aqueous solution, extracts from G. tenuifolia and C. oleifera flowers provide abilities to inhibit matrix metalloproteinases (MMPs), which is able to protect collagen fibers against degradation and leading to anti-ageing. This influence suggests that these three subjects may be considered effective ingredients for cosmetics application. |
