台灣小返魂抗氧化能力分析與小刺肉質軟珊瑚所含化學成分之研究

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標題:	台灣小返魂抗氧化能力分析與小刺肉質軟珊瑚所含化學成分之研究 Studies on antioxidant capacity analysis of the Phyllanthus amarus and chemical constituents from the Formosan soft coral Sarcophyton tenuispiculatum
作者:	李蕙君
貢獻者:	嘉南藥理科技大學：化妝品科技研究所王貴弘
關鍵字:	小返魂抗氧化小刺肉質軟珊瑚 Sarcophyton tenuispiculatum antioxidant Phyllanthus amarus
日期:	2011
上傳時間:	2011-10-25 13:27:58 (UTC+8)
摘要:	本論文第一部分研究小返魂 (Phyllanthus amarus) 之抗氧能力。小返魂屬於大戟科 (Euphorbiaceae) 的植物，為台灣常見之路旁小草。根據以往文獻報導，大戟科植物常具備優異的抗氧化及保肝作用。為瞭解小返魂所具備的抗氧化能力並提昇其萃取液的抗氧化效力，因此以簡單的配比分離 (partition) 與管柱層析 (column chromatography) 方法，尋找具備最優異抗氧化效力的萃取程序，以此條件再選擇適合之萃取方法，提昇小返魂萃取液或層析層應用添加在化粧保養品上。本論文以檢測小返魂丙酮萃取物、水與乙酸乙酯配比分離層及乙酸乙酯層之層析分離層的抗氧化效力。研究結果證實小返魂配比分離的水層、乙酸乙酯層和乙酸乙酯粗分層之第 9、10、11層以及水層具有 80% 以上的清除 DPPH 自由基的效能；在清除 ABTS 自由基的檢測結果，顯示小返魂配比分離的水層、乙酸乙酯層和乙酸乙酯粗分層之第 9-14 層達到 80% 以上的清除率；在總酚含量檢驗結果，乙酸乙酯粗分層第 10-12 具有最高的總酚含量。本研究結果，以科學性的實驗及數據驗證小返魂之抗氧化作用集中在中高極性層，因此以簡單的層析即可獲得優異抗氧化效能的萃取液，去蕪存菁排除不具功效的雜質，將高效能的萃取液添加於化粧保養品中，強化其功能性、技術性與市場競爭力。論文第二部份針對台灣產小琉球海域採集的小刺肉質軟珊瑚 (Sarcophyton tenuispiculatum) 之化學成分及其生物活性進行評估。小刺肉質軟珊瑚以 95% 乙醇萃取之粗萃物，發現對於四種抗氧化能力 (清除 DPPH 自由基、螯合亞鐵離子、清除活性氧自由基及總抗氧化能力) 均顯示出優異的抗氧化能力；經由配比分離後所得之乙酸乙酯層粗萃物，對於人類口腔上皮癌細胞 (SCC25) ，及人類肝癌細胞 (HepG2) 細胞顯示出有良好的抑制作用。本研究共分離出 3 個天然化合物，其中分離出 1 個新化合物 1 和 2 個已知的化合物 2-3 。化合物 1-3 的結構，均是經由物理資料 (熔點、旋光度) 及各類型之圖譜解析 (MS、1D, 2D NMR) 以及相關文獻資料加以比對建立。而化合物 1 的相對立體結構，則可經由 X-ray 單晶繞射方法加以確立。於細胞毒殺活性測試方面，結果顯示出化合物 1 對於人類上皮癌細胞 (A431)、人類口腔上皮癌細胞 (SCC25) 及黑色素瘤細胞 (A375)，都具有良好的細胞毒殺活性。而化合物 2 對人類肝癌細胞 HepG2、He3B和 PLC/PRF/5 也顯示無細胞毒殺活性。 In this study, the first part is to analyse the antioxidant capacity of Phyllanthus amarus. The Phyllanthus amarus belongs to plant of an Euphorbiaceae, for common roadside small grass in Taiwan. In previous studies, the extrace from the plant of Euphorbiaceae were found to show significant antioxidation and protect a liver function. For understanding the ability of the antioxidizes of P. amarus and promoting the effect that the antioxidizes of extract, we use simple partition and column chromatography, look for having excellent extracting method of antioxidize effect. Using this condition select suitable extract method again to promote P. amarus extract. Extract layer application to add in cosmetics products. This paper detect the acetone extract of P. amarus, the antioxidizes effect of chromatography layer of water and Ethylacetate partition layer and ethy lacetate layer. The paper result confirmed P. amarus partition of water layer, ethy lacetate layer and ethy lacetate rough segmentation layer of the 9, 10, 11, water layers have the effect of the free radicals of clearance DPPH of above 80%; the detect result of the rate of the ABTS•+ cation clearance, show P. amarus partition of water layer, ethy lacetate layer and ethy lacetate rough segmentation layer of the 9-14 layers reach the clearance rate of above 80%; The total polyphenol content detect a result, ethy lacetate rough segmentation layer of 10-12 have the highest total phenol content. This paper result confirmed, with the science experiment and the data. Identify P. amarus of the concentration of the antioxygenation effect at in middle and high polarity layers, therefore immediately acquire the extract that the excellent antioxidizes effect by simple chromatography, exclude doesn't have the impurities of effect, the extract of the high performance is added to cosmetics products, enhance its function, technique and the competition ability of market. Secondly, we searched the chemical constituents of the Formosan soft coral Sarcophyton tenuispiculatum, and also assayed other analysis of biological activities. 95% Alcohol crude extracts of S. tenuispiculatum were found to show significant antioxidant capability against DPPH, chelating effect of Fe2+, superoxide anion and total antioxidant capacity. Furthermore ethyl acetate crude extract by the partition that were found to show siginificant activity against SCC25 (human oral squamous cell carcinoma) and HepG2 (human hepatoma). Therefore, this study led to the isolation of three natural products 1-3, including a new compound 1, along with two know compounds.The structures of compounds 1-3 were established by detailed spectral data analysis (MS, 1D and 2D NMR) and by the comparison spectral data with those of the related known compounds. The relative configuration of metabolite 1 was further confirmed by X-ray single-crystal diffraction analysis. In the cytotoxicity test, compound 1 showed significant activity toward the above A431 (human epidermoid carcinoma), SCC25 (human oral squamous cell carcinoma) and A375 (human amelanotic melanoma) Furthermore, compound 2 did not show a significant cytotoxicy against HepG2、Hep3B amd PLC/PRF/5 (human hepatoma).
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顯示於類別:	[化妝品應用與管理系(所)] 博碩士論文

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