Role of Cholesterol in |5-Amyloid Precursor Protein Processing and Oligomerization

CNU IR > Pharmacy and Science > 2011 Taiwanese-Russian Organic, Medicinal and Bio Chemistry Interactions & PST Medicinal Chemistry Symposium > Item 310902800/24264

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Title:	Role of Cholesterol in \|5-Amyloid Precursor Protein Processing and Oligomerization
Authors:	Ke-Ming Huang (黃可名) Fan-Lu Kung
Date:	2011
Issue Date:	2011-06-23 14:57:46 (UTC+8)
Abstract:	The relationship between cholesterol and (β-amyloid precursor protein (APP) processing is an attractive mystery in the Alzheimer's disease (AD) field. It has been hypothesized that there is a direct interaction between cholesterol and APP, which can be processed into Aβ in its pathogenic pathway. To assess the importance of APP-cholesterol interaction in regulating APF processing, we generate several cholesterol-binding-deficient mutant APP proteins via introducing single-site mutations in the putative cholesterol recognition amino acid consensus (CRAC) sequence of APR Our preliminary result reveals that smaller portion of APP-S622L, which has a lower binding affinity for cholesterol than APP wild-type, is co-fractionated with lipid raft marker flotillin-1 compared to wild-type. The C99/C83 ratio is also influenced by this S622L mutation. Further study shows these mutations influence the oligomenzation properties 01 AFF as well, lhese data suggest tne interaction between cholesterol and APP could be a driving force for distributing APP into lipid raft, which makes APP more accessible to the pathogenically critical protein BACE1.
Relation:	2011年台俄有機、藥物與生物化學交流暨藥物化學研討會，起迄日：2011/04/06~2011/04/05，地點：溪頭台大實驗林
Appears in Collections:	[Pharmacy and Science] 2011 Taiwanese-Russian Organic, Medicinal and Bio Chemistry Interactions & PST Medicinal Chemistry Symposium

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