Evaluation of Antioxidant and DNA Protection Activities in the Extracts of Leaves of the Lotus Plant

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Proceedings > Item 310902800/23944

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/23944

题名:	Evaluation of Antioxidant and DNA Protection Activities in the Extracts of Leaves of the Lotus Plant
作者:	Zong-Shiow Chen Ching-Gong Lin Pandora Tsai
贡献者:	化粧品科技研究所
关键词:	Lotus leaf antioxidant DNA protection
日期:	2009-08
上传时间:	2011-06-09 14:59:53 (UTC+8)
摘要:	The lotus plant (Nelumbo nucifera Gaertn.) is not known in Western countries for food consumption. However, for more than 3000 years, lotus has been cultivated as a crop in Far East Asia, for food and medicine. This plant also plays a significant role in religious and cultural activities. The aim of the present study was to examine the antioxidant and DNA protection activities in the extracts from the leaves of the lotus plant. The extract from the lotus leaf was used in this study. Antioxidant activities were measured by both 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH•) scavenging and 2, 2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS•+) decolourisation methods. To further evaluate the effect of lotus extracts on UV induced DNA damages, the DNA protection assay was employed. Lotus leaf extracts had effective DPPH bleaching activity and ABTS•+ radical scavenging activity in a concentration dependent manner. Furthermore, the lotus leaf extracts to inhibit the oxidative DNA damages were assessed by measuring the conversion of supercoiled pUC119 plasmid DNA to the linear forms. UV irradiation of DNA with hydrogen peroxide resulted in the formation of linear forms of DNA, indicating double-strand DNA breaks. Addition of lotus plant extracts to DNA resulted in a partial inhibition of the conversion of supercoiled DNA to linear forms, indicating that the lotus plant extract was able to protect plasmid DNA against hydroxyl radical induced oxidative damage. The inhibition of hydroxyl radical induced DNA strand breaks by lotus plant extracts exhibited a concentration dependent relationship. Similar protective results were also obtained in the l phage DNA (Hind III digested) experiment
關聯:	IUPAC CONGRESS 2009，起迄日：2009/8/2~2009/8/7，地點：Glasgow
显示于类别:	[Dept. of Cosmetic Science and institute of cosmetic science] Proceedings

文件中的档案:

没有与此文件相关的档案.

在CNU IR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....