台灣產柳珊瑚及葉下珠所含天然化合物之研究

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Title:	台灣產柳珊瑚及葉下珠所含天然化合物之研究 Studies on Natural Products of the Formosan Isis hippuris and Phyllanthus urinaria
Authors:	黃惠婷
Contributors:	王貴弘嘉南藥理科技大學：藥物科技研究所
Keywords:	Isis hippuris Phyllanthus urinaria 活性成份分析抗氧化能力(DPPH、ABTS) 總酚定量試驗 Phyllanthus urinaria analysis of active compounds total phenolic contents antioxidant analyses (DPPH、ABTS) Isis hippuris
Date:	2010
Issue Date:	2010-12-30 15:34:12 (UTC+8)
Abstract:	本論文主要之研究目的在於分析從台灣小琉球海域採集的柳珊瑚(Isis hippuris)與台灣葉下珠(Phyllanthus urinaria)之化學成分及其他生物活性的評估。 I. hippuris乙酸乙酯之萃取物發現對於人類頭頸部上皮麟狀癌細胞(SCC-25)顯示出有良好之抑制作用；葉下珠(P. urinaria)丙酮萃取物發現對於Huh7 281 cell(單純人類C型肝癌細胞株)與抗氧化能力(DPPH、ABTS)均顯示出具優異之抑制作用，尤其水層在濃度1000 ppm之DPPH自由基清除率更達95 %以上；在ABTS+陽離子清除率的檢測結果顯示在濃度1000 ppm時乙酸乙酯粗分層第18層之抑制率達95 %以上。總酚定量測試結果顯示乙酸乙酯粗分層第15、18與水層之乾燥萃取物每1克中含有相當於106.6 mg、84.1 mg、76.7 mg之gallic acid。進一步分離出共7個已知化合物。化合物1-7之結構，均是經由物理資料熔點、旋光度及各類型之圖譜解析(MS,1D、2D NMR)以及相關文獻資料加以比對建立。本研究將所獲得的化合物1-3進行各種不同之癌細胞株的細胞毒殺試驗。在細胞毒殺活性測試方面結果顯示出化合物1-3對人類上皮癌細胞(A-431)與人類頭頸部上皮麟狀癌細胞(SCC-25)並沒有顯著之抑制作用。而化合物3對人類直腸癌細胞(SW-480)也顯示出無顯著之細胞毒殺活性。 The main purpose of this is to study the chemical constituents of the Formosan Isididae Isis hippuris and Original Plant Phyllanthus urinaria, as well as analyse their other analysis of biological activities. Ethyl acetate extracts of I. hippuris were found to show siginificant activity against SCC-25(human oral squamous cell carcinoma). Furthermore, Acetone extracts of P. urinaria were found to show moderate activity against Huh7 281 cell (human hepatocellular carcinoma) and significant antioxidant capability against DPPH and ABTS+. In particular, water layer at a concentration of 1000 ppm in the DPPH demonstrated a radical scavenging rate of more than 95 %. In ABTS cation, the results of the radical scavenging effect show that EtOAc layer at fraction 18 of the concentration of 1000 ppm was inhibition effect of more than 95 %. Total phenols quantitative test results indicate that EtOAc layer at fraction 15, 18 and water layer contained a level of total phenols mg/g dry plant extract (in Gallic acid) of 106.6 mg、84.1 mg、76.7 mg, respectively. Therefore, this study led to the isolation of seven know natural compounds. The structure of compounds 1-7 were established by detailed spectral data analysis (MS, 1D and 2D NMR) and by comparison of the spectral data with those of the related known compounds. The cytotoxicity of compounds 1-3 against different cancer lines were determined. In the cytotoxicity test, results show compounds 1-3 do not possess cytotoxicies against A-431(Human epidermoid carcinoma) and SCC-25(Human oral squamous cell carcinoma) cell lines. Furthermore, compound 3 did not show a significant cytotoxicy against SW-480 (Human colon carcinoma).
Relation:	校內校外均不公開，學年度：98，118頁
Appears in Collections:	[Dept. of Pharmacy] Dissertations and Theses

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