固態桑黃之體內體外免疫調功效

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Title:	固態桑黃之體內體外免疫調功效 Study in vitro of in vivo on the immunomodulatory properties of solid cultured Phellinus linteus
Authors:	楊倖嘉
Contributors:	邢中熹吳淑靜嘉南藥理科技大學：營養與保健科技研究所
Keywords:	固態培養免疫調節多醣桑黃 immunomodulatory solid cultured polysaccharide Phellinus linus
Date:	2010
Issue Date:	2010-12-30 09:48:29 (UTC+8)
Abstract:	桑黃 (Phellinus linteus)（PL）一般認為具有抗發炎、保肝及免疫調節及其他療效。然而，有關固態桑黃之體內及體外免疫特性及其分子作用機制仍不清楚。本研究的目的是(1)檢測探討固態培養桑黃(水及95%酒精)萃取物及多醣(SCPLP)對細胞存活率；(2)證實桑黃多醣(SCPLP)在細胞經由脂多醣 (LPS) 誘導人類單核球細胞（THP-1）之MAPK 訊息路徑、COX-2 的系統（COX-1 及COX-2）及iNOS，Catalase 及GPX。(3) 研究桑黃多醣(SCPLP) 對非特異性，如脂多醣 (LPS) 誘導之細胞激素(IL-6、MIP-2 及TNF-α)形成，腹腔巨噬細之胞吞噬作用及一氧化氮(NO)之形成；以及特異性，如OVA 誘導免疫球蛋白IgE 及IgG 之形成及遲發性過敏反應-掌蹼試驗之影響。XTT 試驗結果顯示，固態培養桑黃所分離之多醣（SCPLP）比其他萃取物(水及 95%乙醇)對LPS-誘導之THP-1 細胞具有更好的細胞存活率。在5.0 μg/ml SCPLP 明顯抑制LPS 誘導所引起的iNOS 和COX-2 和Catalase 之表現，並能抑制了磷酸化JNK 和磷酸化p38 的活化。但COX-1，ERK 及p-ERK 則沒有顯著的表現。另一方面在動物實驗結果顯示，非特異性免疫反應實驗中100 mg/kg/day SCPLP 可有效降低一氧化氮(NO)之形成，及具有劑量效應的調節TNF-α，IL-6 及MIP-2 的表現，對LPS 誘導之血清或腹腔巨噬細胞。當BALB/c 小鼠經口管餵50 mg/kg/day SCPLP 14 天後，可明顯降低左、右掌蹼之腫脹，尤其在48 及72 小時。經口管餵SCPLP 可顯著降低由OVA 誘導之IgG 及IgE 之表現量。這些結果顯示桑黃多醣(SCPLP)，未來可發展為具有潛力的一種免疫調節物質。 Phellinus inteus (PL) is generally believed to possess anti-inflammatory, hepatoprotective, immunomodulatory and other therapeutic properties. However, in vitro and in vivo immunomodulatory properties of solid cultured P. linteus and its molecular mechanism(s) of action remain unknown. The aims of this study were (1) to examine the effects of solid cultured P. linteus extracts (water and 95%ethanol) and polysaccharide on cell viability；(2) to confirm of SCPLP on the MAPK signaling pathway and COX-2 system(COX-1 and COX-2)，iNOS，Catalase and GPX in LPS-induced human monocytic cells (THP-1) ； and (3) to study the effects of SCPLP in non-specific (i.e. lipopolysaccharide (LPS)-induced cytokines formation，phagocytosis and ntric oxide (NO) generation and specific (i.e. OVA-induced specific antibodies such as IgG and IgE generation and footpad swelling effects.) The MTT assay showed that SCPLP possessed a better cell viability than other extracts in LPS-induced THP-1 cells. At 5.0 μg/ml，SCPLP significantly blocked the LPS induction of iNOS，COX-2 and GPX expression，suppressed the actiyation and phosphorylation of JNK and p38，but not COX-1，Catalase，ERK and p-ERK. Animal result showed that SCPLP 100mg/kg/day effectively decreased the generation of NO，and dose-dependently down-regulated the TNF-α，IL-6 and MIP-2 expreaaion in the LPS-induced serum or peritoneal macrophages. Mice fed 50mg/kg/day of SCPLP for 14 days displayed a significantly (P<0.05) lesser left or right footpad swelling at 48 and 72 h. Oral administration of SCPLP significantly decreased the expression of anti-OVA IgG and anti-OVA IgE levels OVA-immunization. These results that SCPLP could be developed as a potential immunomodulator.
Relation:	校內校外均不公開，學年度：98，72頁
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Dissertations and Theses

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