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    Title: Supercritical carbon dioxide extract of Physalis peruviana induced cell cycle arrest and apoptosis in human lung cancer H661 cells
    Authors: Shu-Jing Wu
    Shun-Pang Chang
    Doung-Liang Lin
    Shyh-Shyan Wang
    Fwu-Feuu Hou
    Lean-Teik Ng
    Contributors: 保健營養系
    Keywords: Physalis peruviana
    Supercritical carbon dioxide extraction
    Cell cycle arrest
    Date: 2009-06
    Issue Date: 2010-11-12 16:11:30 (UTC+8)
    Abstract: Physalis peruviana L. (PP) is a popular folk medicine used for treating cancer, leukemia, hepatitis, rheumatism and other diseases. In this study, our objectives were to examine the total flavonoid and phenol content of different PP extracts (aqueous: HWEPP; ethanolic: EEPP; supercritical carbon dioxide: SCEPP-0, SCEPP-4 and SCEPP-5) and their antiproliferative effects in human lung cancer H661 cells. Among all the extracts tested, results showed that SCEPP-5 possessed the highest total flavonoid (226.19 � 4.15 mg/g) and phenol (100.82 � 6.25 mg/g) contents. SCEPP-5 also demonstrated the most potent inhibitory effect on H661 cell proliferation. Using DNA ladder and flow cytometry analysis, SCEPP-5 effectively induced H661 cell apoptosis as demonstrated by the accumulation of Sub-G1 peak and fragmentation of DNA. SCEPP-5 not only induced cell cycle arrest at S phase, it also up-regulated the expression of pro-apoptotic protein (Bax) and down-regulated the inhibitor of apoptosis protein (IAP). Furthermore, the apoptotic induction in H661 cells was found to associate with an elevated p53 protein expression, cytochrome c release, caspase-3 activation and PARP cleavage. Taken together, these results conclude that SCEPP-5 induced cell cycle arrest at S phase, and its apoptotic induction could be mediated through the p53-dependent pathway and modification of Bax and XIAP proteins expression. The results have also provided important pharmacological backgrounds for the potential use of PP supercritical fluid extract as products for cancer prevention.
    Relation: Food and Chemical Toxicology 47(6):p.1132-1138
    Appears in Collections:[Dept. of Health and Nutrition (including master's program)] Periodical Articles

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