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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/23173

    標題: 黑鯛第一型類胰島素生長因子基因片段之合成
    Amplification of Black Seabream Insulin-Like Growth Factor I Dna by Polymerase Chain Reaction
    作者: 吳鴻程
    貢獻者: 食品衛生科
    關鍵字: 黑鯛
    black seabream
    polymerase chain reaction
    isulin-like growth factor I
    DNA primer
    日期: 1994
    上傳時間: 2010-11-10 18:22:10 (UTC+8)
    摘要: 依據黑鯛第一型類胰島素生長因子(Insulin-like growth factor I, IGF-1)DNA序列,合成長度為23核苷酸的S核苷引子,19個核苷酸的B核苷引子,21個核苷酸的D核苷引子和22個核苷酸的E核苷引子。配對後的核苷引子利用Vent核苷聚合酶進行連鎖反應;其反應條件為:解鏈溫度94℃(反應1分鐘),黏合溫度65℃(反應2分鐘),合成溫度72℃(反應2分鐘),共重複35次循環。經核苷酸聚合酶連鎖反應後,可生成B區域到D區域201個核苷酸DNA片段;B區域到E區域423個核苷酸的DNA片段,S區域到E區域552個核苷酸的DNA片段。S區域到D區域的DNA片段,則在解鏈溫度94℃(反應30秒),黏合溫度54℃ (反應30秒),合成溫度74℃(反應30秒),共進行35次循環的條件下可生成330個核背酸之DNA片段。
    Four oligonuclotide primers, primer S (23 bases), primeer B (19 bases), primer D (21 bases)and primer E (22 bases), were synthesized according to the black seabream (Acanthopagrus schegeli) insulin-like growth factor I (IGF-1) DNA sequence to amplify the IGF-1 DNA fragments.The results found that primeer S, primer E produced 552 base pairs IGF-1 DNA fragment, primer B, primer D producted 201 base pairs IGF-1 DNA fragment, and primer B, primer D produced 423 base pairs IGF-1 fragment at denatured 1 minute at 94℃, annealized 2 minutes at 65℃, and extended 2 minutes at 72℃ by polymerase chain reaction. Primer S and primer D producted 330 base pairs JGF-1 DNA fragment at denatured 30 seconds at 94℃, annealized 30 seconds at 54℃ and extended 30 seconds at 74℃ by polymerase chain reaction.
    關聯: 嘉南學報 20期:p.138-147
    Appears in Collections:[嘉南學報] 20期 (1994)
    [ 食品科技系 ] 期刊論文

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