Comparative proteomics on resting and activated human basophilic leukemic KU812 upon stimulation with PMA plus A23187

Chia Nan University of Pharmacy & Science Institutional Repository > 智慧生活學院 > 生活保健科技系 > 期刊論文 > Item 310902800/23147

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標題:	Comparative proteomics on resting and activated human basophilic leukemic KU812 upon stimulation with PMA plus A23187
作者:	Tai-Yuan Chen Ya-Hui Lu Jen-Yu Li Chia-Jung Chen Wei-Humg Hsu Hong-Yu Lin Deng-Fwu Hwang Shui-Tein Chen
貢獻者:	生活應用與保健系
關鍵字:	basophil proteomics immuno-stimulants Th2 cytokine
日期:	2009-10
上傳時間:	2010-10-27 16:27:34 (UTC+8)
摘要:	Basophil activation has been implicated in allergy reaction and immune modulation. Signaling transduction, mediator release and morphology change of basophils have been largely deciphered. This study examined the feasibility of using proteomic strategy to investigate the whole cell proteomic changes in KU812 basophils following activation with phorbol myristate acetate (PMA) plus ionophore (A23187). The two-dimensional electrophoresis (2D) coupled with matrix-assisted laser desorption/ionization-quadrupole/time-of-flight (MALDI-Q-TOF) was selected for studying comparative proteome differences; and cytokine mRNA expressions were monitored. The differentially displayed proteins were further validated by 1D-and 2D-Western blotting for protein expressions. IL-4, IL-5, and IL-13 mRNAs in KU812 were significantly induced; a phenomenon similar to the allergy reaction. The protein expressions of heterogeneous nuclear ribonucleoprotein K (HNRPK) and a-enolase (ENO1) were shown a good correlation according to the results from the 2D, and 2D-Western blots. Since the majority of the identified proteins were associated with immunological, inflammatory disorders and tumor genesis, this study in defining the proteomic changes in basophilic cells that occur in response to treatment with PMA plus A23187 can serve as biomarkers for characterization of in vitro immuno-stimulation.
關聯:	Journal of Food and Drug Analysis 17(5)：p.363-375
显示于类别:	[生活保健科技系] 期刊論文

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