Noninvasive optical imaging for tracking gene delivery and recombination in tumor

doi:10.4015/S1016237209001441

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/23142

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/23142

Title:	Noninvasive optical imaging for tracking gene delivery and recombination in tumor
Authors:	Mei-Ru Chen Vivia Yu-Ying Kao Shan-Wen Liu Tzu-Chin Wu Hsiao-Chi Yu Chin-Han Hsu Chin-Tu Chen Kurt M. Lin
Contributors:	生物科技系
Keywords:	Green fluorescence protein Bioluminescence Firefly luciferase Cre recombinase loxP site
Date:	2009-10
Issue Date:	2010-10-27 14:14:29 (UTC+8)
Publisher:	World Scientific Publ Co Pte Ltd
Abstract:	Here, we report the generation of optical imaging reporter breast tumor cells that allow the longitudinal, in vivo, noninvasive imaging of gene recombination in tumor. Tumor-gene targeting is a promising approach of treating cancers, and a suitable gene delivery method is the criteria for success. By using the cre lox genetic engineering tool, we targeted stable green fluorescent protein expression in metastatic-prone human breast cancer MDA-MB231 cells that switch to express firefly luciferase upon the exogenous delivery and expression of cre DNA recombinase. We tested this model in vivo by intratumor injection of cre adenovirus and demonstrated the usefulness of this model to achieve longitudinal bioluminescence imaging of DNA recombination in tumor. This optical imaging vector and tumor model will facilitate the research for biomaterial solutions for carriers in gene therapy, and in studies on tumor targeting, tracking for tumor metastasis and migration of tumor stem cells, and for determining the anticancer drug efficacy.
Relation:	Biomedical Engineering Applications, Basis and Communications 21(5)：p.355-360
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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