The characteristics and transfection efficiency of cationic poly (ester-co-urethane) – short chain PEI conjugates self-assembled with DNA

doi:10.1016/j.biomaterials.2009.08.052

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Periodical Articles > Item 310902800/23134

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题名:	The characteristics and transfection efficiency of cationic poly (ester-co-urethane) – short chain PEI conjugates self-assembled with DNA
作者:	Xin-Yi Liu Wen-Yueh Ho Wei Jing Hung Min-Da Shau
贡献者:	化粧品應用與管理系生物科技系(所)
关键词:	Transfection poly(ester–co–urethane) Poly(ethylenimine) Buffering capacity
日期:	2009-12
上传时间:	2010-10-26 16:07:00 (UTC+8)
摘要:	To improve the transfection efficiency of polycations with DNA, we synthesized poly(ester–co–urethane)(PEU-g-PEI800) with short chain PEI800 in the side chain, and poly(ester–co–urethane)(PEU) without short chain PEI800. Both PEU-g-PEI800 and PEU, readily self-assembled with plasmid DNA (pCMV-βgal) in a HEPES buffer, were characterized by dynamic light scattering and zeta-potential. The results reveal that PEU-g-PEI800 and PEU were able to self-assemble particles with DNA and yield nano-sized complexes (<200 nm) with positive charge at N/P ratios of 20/1 and 120/1, respectively. The degradation studies indicate that the half-life of PEU-g-PEI800 and PEU in the HEPES buffer were 14 and 35 h at pH 7.4, respectively. Titration studies were performed to determine the buffering capacities of the polymers. The COS-7 cell viabilities in the presence of PEU-g-PEI800/DNA, PEU/DNA, and PEI25k/DNA were studied. In addition, The PEU-g-PEI800/DNA complexes were able to transfect COS-7 cells in vitro with a high efficiency comparable to a well-known gene carrier PEI25k. The results indicate that PEU-g-PEI800 is an attractive cationic poly (ester–co–urethane) for gene delivery and an interesting candidate for further study.
關聯:	Biomaterials 30(34)：p.6665-6673
显示于类别:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles [Dept. of Biotechnology (including master's program)] Periodical Articles

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