Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/23130
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 18268/20495 (89%)
Visitors : 8619590      Online Users : 691
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/23130


    题名: Effect of chain length on physicochemical properties and cytotoxicity of cationic vesicles composed of phosphatidylcholines and dialkyldimethylammonium bromides.
    作者: Chia-Hua Liang
    Tzung-Han Chou
    贡献者: 化粧品應用與管理系
    关键词: Cationic vesicles
    Phosphatidylcholines
    Dialkyldimethylammonium bromides
    Physicochemical characteristics
    Cytotoxicity
    Apoptosis
    日期: 2009-04
    上传时间: 2010-10-26 16:06:50 (UTC+8)
    出版者: Elsevier Ireland Ltd
    摘要: This study investigated the physicochemical characteristics of cationic vesicles that were prepared from two phosphatidylcholines and three dialkyldimethylammonium bromides (DXDAB) with differing in dialkyl chain lengths, ranging from 2-C(14) to 2-C(18), by measuring particle size and zeta potential. The dependence of particle size, zeta potential and short-storage stability of mixed phosphatidylcholine/DXDAB vesicles on the chain length and composition were also elucidated. Transmission electron microscopy analysis verified that vesicles were formed as a phosphatidylcholine film to which DXDAB was added in a phosphate buffer saline (PBS, pH 7.4). Furthermore, the toxicity to the human keratinocytes (HaCaT) and squamous cell carcinomas (SCC25) cells that were incubated with these vesicles, evaluated by a cell viability assay, increased with the percentage of DXDAB that was incorporated and was inversely proportional to the chain length of DXDAB. The morphological features (round shape, chromatin condensation and apoptosis bodies) and results of flow cytometry analysis (increased sub-G(1) fraction) confirmed the induction of apoptosis in HaCaT and SCC25 cells by cationic vesicles. Apoptosis caused by cationic vesicles without the addition of any drugs was observed for the first time in HaCaT and SCC25 cells. The results of this investigation suggest that cytotoxicity is related to the zeta potential of the cationic vesicles.
    關聯: Chemistry and Physics of Lipids 158(2):p.81-90
    显示于类别:[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

    文件中的档案:

    档案 描述 大小格式浏览次数
    index.html0KbHTML2598检视/开启


    在CNU IR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback