Tyrosinase Inhibitors isolated from the roots of Paeonia suffruticosa

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Title:	Tyrosinase Inhibitors isolated from the roots of Paeonia suffruticosa
Authors:	Hsiou-Yu Ding Hang-Ching Lin Te-Sheng Chang
Contributors:	化粧品科技研究所
Date:	2009-05
Issue Date:	2010-10-26 16:01:00 (UTC+8)
Abstract:	The inhibition of mushroom tyrosinase by Paeonia suffruticosa root-derived materials was evaluated. Six tyrosinase inhibitors were isolated by ethanol extraction, n-hexane, ethyl acetate, n-BuOH, and water partition, silica gel column chromatography, Sephadex LH-20, Lobar PR-8, and high-performance liquid chromatography methods, and they were identified as kaempferol (I), quercetin (II), mudanpioside B (III), benzoyloxypaeoniflorin (IV), mudanpioside H (V), and pentagalloyl-β-D-glucose (VI) on the basis of spectroscopic evidence. The inhibitory activities of compounds I to VI against mushroom tyrosinase were determined with IC50 values of 0.120, 0.108, 0.368, 0.453, 0.324, and 0.063 mM, respectively. The kinetic study indicated that all purified inhibitors acted competitively for the L-dopa binding site of the enzyme, with an exception of compound VI, which acted non-competitively.
Relation:	Journal of Cosmetic Science 60：p.347–352
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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