English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17744/20032 (89%)
Visitors : 7230208      Online Users : 319
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/23089

    標題: Alendronate與Teriparatide對股骨頭壞死之崩塌預防-大鼠動物實驗
    Alendronate and Teriparatide for preventing collapse of osteonecrosis of the femoral head – experiment using a rat model
    作者: 董耀天
    貢獻者: 李國榮
    關鍵字: 動物模型
    animal model
    osteonecrosis of femoral head
    日期: 2010
    上傳時間: 2010-10-12 15:10:52 (UTC+8)
    摘要: 股骨頭壞死成因非常多,現所知引起相關危險因子有:類固醇或酗酒因促使凝血機制中脂肪細胞之堆積而引發引發局部之體積與壓力差改變而引發失調為主。在歐美地區,股骨頭壞死其盛行率佔人工關節手術病患之10%(9,23);但在亞洲人之資料發現,其佔人工關節手術病患 20%~25%等。尤其是台灣地區,股骨頭壞死佔人工關節手術病患之比率為 50%。
    實驗設計:用8~10週的大鼠,每週三次腹腔注射:methylprednisolone (40 mg /day),總共六次注射高劑量類固醇。類固醇藥品注射後,使用動物用跑步機後會有近八成大鼠會有股骨頭壞死之症狀。經X光確認大鼠有骨壞死相關症狀且尚未崩塌前,將其分成兩組藥品治療,Alendronate 以0.015g/kg/day或 Teriparatide 20μg/day 治療,以評估Alendronate & Teriparatide 兩組藥物對股骨頭壞死效果與生理變化。結果發現:1. 在活動力方面:股骨頭壞死之大鼠以Alendronate 治療後比Teriparatide 治療後之大鼠明顯活動力比較強,但都比注射生理食鹽水之大鼠活動力弱。2. 在顯微鏡下,大鼠股骨頭中之生長板上明顯在methylprednisolone 加上動物用跑步機處理使其股骨頭壞死且股骨頭內之生長板到其股骨頭外緣距離明顯萎縮,但經過Alendronate 或 Teriparatide 之治療後,發現其兩者相差之距離明顯回復到與生理食鹽水組別相似。此結果亦證實對於股骨頭壞死之大鼠,使用Alendronate & Teriparatide 兩者對於股骨頭壞死有相當之治療效果,且有機會回復到正常狀況。
    Osteonecrosis (Avascular necrosis, Aseptic necrosis) of the femoral head is the most common illness among young to mid-age population in Taiwan. Treatment options of osteonecrosis depend on the stage and percentage of the necrotic area. Salvage of the femoral head is the top priority before collapse. It has been show that in both animal study and clinical trial proved that bisphosphonate compounds reduce and delay deformity of the necrotic femoral head. Besides, teriparatide, a synthetic parathyroid hormone(PTH) and PTH analog, stimulates bone formation and inhibits bone destruction. We have created an animal model of osteonecrosis of the femoral head with high dose of corticosteroids treatment, followed by treadmill running in Sprague-Dawley rats, which mimicks the osteonecrosis of human. This animal model can be applied for the testing of the therapeutic effects of bisphosphonate and teriparatide.
    Materials and Methods:
    42 Spague-Dawley rats weighing 300-350 grams were performed in this study. They were divided into four groups. Control group contained 16 rats and each received intraperitoneal injection of 1 ml normal saline every other day for 2 weeks.In experimental group, each rat received 40 mg/kg methylprednisolone peritoneal injection every other day for two weeks. All the rats underwent treadmill running (8-10 rpm, 18 degree upward slope)for 10 to 15 minutes per day from the third to the sixth week. After treadmill running, each rat were examined and only rats developed osteonecrosis were randomly assgned to receive either alendronate 0.015mg/kg/day intraperitoneal injection or teriparatide 20 mcg/day intramuscular injection for 4 weeks or no treatment.
    Gross deformity was identified in 80 % of the retrieved femoral heads in the all of rats by gross inspection and X-ray examination. Histological examination of theses femoral heads confirmed deformity(collapse) of the femoral head. The distance between the physis to the joint cartilage reduced significantly, in non-treatment. Howere, treatment with either teriparatide or alendronate restored the distance shortage between physis to joint cartilage.
    Conclusion and Significance:
    In this study we have provided evidence showing that high dose of corticosteroid treatment and treadmill running result in the collapse of the femoral which mimicks pathogenesis of osteonecrosis of the human. Both alendronate and teriparatide inhibit the deformity of the femoral head comparing with the animals without treatment. This result suggests that this model system can be applied for further screening of possible treatment of osteonecrosis of the femoral head.
    關聯: 校內外均一年後公開 ,學年度:98,90頁
    Appears in Collections:[生物科技系(所)] 博碩士論文

    Files in This Item:

    File Description SizeFormat

    All items in CNU IR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback