Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/23066
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    標題: 繖型科生藥萃取物之抗氧化能力研究
    Studies on the antioxidant capacity of extracts From Umbelliferae Natural medicines
    作者: 蔡玉敏
    貢獻者: 王貴弘
    嘉南藥理科技大學：化妝品科技研究所
    關鍵字: 八種繖型科生藥
    TPC
    ABTS
    DPPH
    抗氧化
    Umbelliferae Natural medicines
    TPC
    antioxidant
    DPPH
    ABTS
    日期: 2010
    上傳時間: 2010-10-08 13:41:45 (UTC+8)
    摘要: 本研究以生活當中較常被國人使用於抗炎、止痛保健之藥材，歸納出八種繖形科生藥白芷Angelica dahurica(Fisch.ex Hoffm.) Benth.et Hook.f.、川芎Ligusticum chuanxiong Hort、藳本Ligusticum sinensis Oliver、防風Ledebouriella seseloides Wolff、當歸Angelica sinensis Diels、羌活Notopterygium incisium Ting、小茴香Cnidium officinale Makino、蛇床子Cnidium monnieri（L.）Cuss粉末，分別以95%乙醇連續提取2次後，收集提取液進行減壓濃縮，得到粗提取物，將所得之提取物分別以水及乙酸乙酯進行配比分離，分別得到水層及乙酸乙酯層。再將每種生藥之不同層的提取物進行抗氧化能力評估，檢測清除DPPH自由基、清除ABTS陽離子自由基之測驗，並利用Folin-Ciocalten試劑來分析八種繖形科生藥之水層與乙酸乙酯層配比分離後的總酚類含量。
    結果顯示本研究之8種繖型科生藥之不同溶劑之萃取物均具有自由基清除能力。結論如下：
    1.羌活(Ni)萃取物濃度在400 ppm時有明顯之抗氧化力，其乙酸乙酯層之ABTS陽離子自由基清除力高達89.4% (IC50為213.1 μg/mL)，乙醇萃取物亦有57.6%之清除力；對DPPH自由基的清除力達53.9%。
    2.川芎(Lc)的乙酸乙酯層配比分離物在ABTS陽離子自由基有很強之清除力，在400 ppm時清除力高達92.5%，200 ppm時達58.7%。
    3.當歸(As)的乙酸乙酯層配比分離物在400 ppm有良好的抗氧化力表現，對ABTS陽離子自由基清除力達94.7% (IC50為175.4 μg/mL)，對DPPH自由基的清除力亦達54.8%。
    4.白芷(Ad)在400 ppm時清除ABTS陽離子自由基能力，乙酸乙酯層配比分離物達70.7% (IC50為263.1 μg/mL)。
    5.蛇床子(Cm)之乙醇及水層配比分離物在400 ppm時對ABTS陽離子自由基之清除力達48.7%、40.4%。
    6.小茴香(Co)在高濃度時雖無明顯之抗氧化力(僅在乙酸乙酯層配比分離物對ABTS陽離子自由基有50.9%之清除率，IC50為375.5 μg/mL)，但在濃度25~100 ppm間各種溶劑萃取物之抗氧化力皆非常平穩。
    7.防風(Lse)僅在乙酸乙酯層配比分離物400 ppm時對ABTS陽離子自由基之清除力達57.8% (IC50為324.3 g/mL)，其他方面則無特殊表現。
    8.藁本(Lsi)僅在EA層配比萃取物400 ppm時對ABTS陽離子自由基有良好之清除力達95% (IC50為195.4 μg/mL)，其他方面亦無特殊表現。
    期待藉由本研究能提供各樣品之有效濃度及萃取率，做為產品添加上的參考。
    This study focuses on eight commonly used Umbelliferae natural medicines which have anti-inflammatory and analgesic effects for our health in daily life,including Angelica dahurica(Fisch.ex Hoffm.) Benth.et Hook.f.、Ligusticum chuanxiong Hort、L. sinensis Oliver、Ledebouriella seseloides Wolff、A. sinensis Diels、Notopterygium incisium Ting、Cnidium officinale Makino and C. monnieri powder.
    These natural medicines are extracted twice with 95% ethanol before collecting the extracted liquid and evaporating it under vacuum,thereby obtaining the crude extract and partitioning it with water and ethylacetate. Then the layer of water and layer of ethylacetate are obtained. This study estimates the ability of anti-oxidation of each extract from different layers of Chinese natural medicines, testing the DPPH free radical removal and scavenging effect of ABTS radical cation. Furthermore, Folin-Ciocalten was used to analyze the quantity contained of phenols in the extracts from the layers of water and of ethylacetate from these eight Umbelliferae natural medicines.
    The results show that different solvents of all these eight Umbelliferae herbs listed below the ability to remove free radicals. As the following shown:
    1. The ability of anti-oxidation is significant when the density of Ni’s extract reaches 400 ppm. The ability of scavenging ABTS radical cation partitioned from layer of ethylacetate is as high as 89.5% (IC50 is 213.1 g/mL), and the one from the extract of ethanol is also 57.6%; the DPPH free radical removal is 53.9 %, and contains quite a large quantity of extracts.
    2. Lc’s partitioned from the layer of ethylacetate has a strong ability of scavenging free radical cation, especially when the density reaches 400 ppm where it becomes 99.6% compared with 58.7% when 200 ppm.
    3. As’ partitioned from layer of ethylacetate performs pretty well on anti-oxidation when it’s at 400 ppm, and the ability of scavenging ABTS free radical cation is 94.7% (IC50 is 175.4 g/mL),and the ability of DPPH free radical removal also reaches 54.8%.
    4. Ad has the ability of scavenging ABTS free radical cation when its density reaches 400 ppm. The extract partitioned from layer of ethylacetate and of the water is 70.7% (IC50 is 263.1 g/mL) and 50%, respechiely,and the extract from ethanol is 54.4%.
    5. Cm: When the density of the extract from ethanol and the one partitioned from layer of water reaches 400 ppm, the ability of scavenging ABTS free radical cation is 48.7% and 40.4%, respechiely.
    6. At a high density, Co has no apparent ability of anti-oxidation (it merely shows a 50.9% ability of scavenging ABTS free radical cation, IC50 is 375.5 g/mL), but extract of every kind of solvent has stable ability of anti-oxidation.
    7. Lse only shows a 57.8% ability of scavenging ABTS free radical cation when the extract partitioned from layer of ethylacetate is 400 ppm (IC50 is 324.3 g/mL) Other than this,there is nothing special.
    8. Lsi shows excellent ability to scavenge ABTS free radical cation (97.2%) when the extract partitioned from layer of ethylacetate is 400 ppm but nothing other than that, either.
    The effectire density & rate-of-extract that are provided of each sample are anticipated to be used as references for commercial purposes.
    關聯: 校內一年後公開，校外永不公開，學年度：98，130頁
    显示于类别:[化妝品應用與管理系(所)] 博碩士論文

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