| Abstract: | 本研究探討豆乳之乳酸菌發酵產物(soymilk-fermented products, SFP)對老鼠3T3纖維母細胞的影響，分析細胞存活率、處理期間膠原蛋白含量的變化、及基質金屬蛋白酶(matrix metalloproteinases, MMPs)酵素活性及酵素分泌量的改變，並評估SFP的抗氧化能力，進一步將SFP調製成化粧品乳液劑型進行人體的有效性試驗。
實驗數據顯示，SFP (3.13 mg/ml、1.56 mg/ml、0.78 mg/ml) 在48小時之處理期間對於3T3纖維母細胞無細胞毒性，而且具有至少30%的促進細胞增生的能力。另外，SFP無法直接抑制細胞培養液所含MMP-2及MMP-9的酵素活性。另一方面，以3.13 mg/ml 的SFP處理纖維母細胞48小時與控制組相較之下，膠原蛋白的累積量可增加15.11%；並且在8小時內能有效促進膠原蛋白累積量的增加。在抗氧化試驗方面，SFP於20 mg/ml時，其清除DPPH之效力可達40%以上。
在人體有效性評估試驗方面，臉部測試部位塗抹含3.44 mg/ml SFP的乳液時，明顯減少受試者的斑點、毛孔、皺紋、粗糙、紫外線斑、及黑色素。四週內平均降低毛孔數29.36％，皺紋14.59％，粗糙度20.36％，紫外線斑1.57％、黑色素9.99％。
由體外試驗分析結果與產品於人體實際使用效果之差異性，顯示SFP可提供商業上化粧品的良好應用。
The objective of this study is to investigate the effects of the products of soymilk fermented with lactic acid bacteria on the cultured mouse 3T3 fibroblasts, and the assays, including cell viability, the level of collagen accumulation, and the activities of matrix metalloproteinases (MMPs) secreted from 3T3 fibroblasts, were performed. The anti-oxidative effect of soymilk-fermented products (SFP) was also evaluated. Besides, the efficacy of lotion containing SFP was further assayed in vivo.
The date presented here showed that 48 h-treatment of 3T3 fibroblasts in the culture medium with 0.78~3.13 mg/ml of SFP could bring about at least a 30% increase in cell proliferation. In addition, SFP (3.13 mg/ml) could not directly inhibit the activities of MMP-2 and MMP-9 in the presence of culture medium. However, it also found that 48 h-treatment of 3T3 fibroblasts with 3.13 mg/ml of SFP could increase the lever of collagen accumulation by 15.11%. Furthermore, within 8h, SFP could effectively give rise to an accumulation of the content of collagen. In antioxidation, 20 mg/ml of SFP showed the scavenging effects on DPPH free radicals over 40%.
On the other hand, in the in-vivo efficacious evaluation, the lotion containing 3.44 mg/ml of SFP dramatically decreased pore counts, wrinkles, textures, UV spots, and melanin by 29.36%, 14.59%, 20.36%, 1.57%, and 9.99%, respectively, in 4 weeks.
Based on the fore-mentioned data, we suggest that SFP can be used as a functional cosmetic ingredient. |
