牙齒美白試劑對人類口腔細胞的影響

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標題:	牙齒美白試劑對人類口腔細胞的影響 The Effect of Tooth Bleaching Drug on Humans Oral Mucosa Cell
作者:	邱政憲
貢獻者:	王四切鍾景宏嘉南藥理科技大學：藥物科技研究所
關鍵字:	氧化壓力粒線體拷貝數目牙齒美白粒線體DNA 4977 bps 斷裂 tooth bleaching mitochondrial mtDNA4977 deletio
日期:	2009
上傳時間:	2010-06-09 09:34:14 (UTC+8)
摘要:	近年，民眾為了擁有健康與燦爛的笑容使得美白牙齒越來越盛行。牙齒美白方法可分為診間美白與居家美白。居家美白在1989年由Haywood 與 Heymann所提出，每晚在病人牙托裡放入10~15 % carbamide perox ide 連續配戴幾個禮拜。與唾液或水接觸時， 10~15 % carbamide peroxide可以分解成3.6~5.4% H2O2 及 6.4-9.6% carbamide (urea)。H2O2不僅為美白成分，同時也為強氧化劑與氧化性物質，會分解成自由基滲透到象牙質與琺瑯質破壞色素達到美白效果。因此我們將探討美白試劑對人類口腔細胞產生的影響。本實驗分成in vitro與in vivo。在in vitro部份反應1與8小時之後，觀察到分別在5 mM 和0.01 mM H2O2會明顯造成hNOK細胞生存率、ROS含量、GSH、粒線體膜電位下降；LDH釋出、核DNA氧化傷害、細胞凋亡情況上升。粒線體copy number無伴隨濃度呈現相關性(1小時, r =－0.395； 8小時，r =－0.223)。另一方面，mtDNA4977程度則隨著時間與濃度相關性越大(1小時, r =0.3728；8小時，r =0.873)，與核DNA氧化傷害也呈現正相關(1 小時, r=0.606；8小時,r=0.863)。在in vivo，24位受試者連續使用美白試劑1個月，每天於睡前配戴8小時。mtDNA4977使用前與使用後1小時、第1、7、14、30天具有統計意義(P<0.05)，停用後第30天則無。copy number 也不具統計意義。因此，牙齒美白試劑所造成的氧化壓力會造成人類口腔細胞不同程度的影響，甚至造成粒線體DNA的變化。將來希望本研究之結果能與臨床資料結合，在既不傷害大量傷害人類正常口腔細胞下，挑擇適當的濃度來進行牙齒美白，不僅可以達到美白效果又達到安全範圍。 Due to the popular demanding of beauty smile, tooth whitening has become a trend in recent years. There are two techniques that dentists use to whiten vital teeth, “in-office bleeching”and “at-home bleeching”. In this study, we focused mainly on home bleeching technique. Home bleaching was first described and published in 1989 by Haywood and Heyman. They put 10-15 % carbamide peroxide in patient-made mouthguard and require users to wear them at night for several weeks. When carbamide peroxide contacts with water or saliva, about 10-15% of carbamide peroxide is degraded to 3.6-5.4% H2O2 and 6.4-9.6% carbamide(urea). H2O2 not only is a bleaching component but also a strong oxidant and oxidizing substance that can break down into free radicals. These free radicals will diffuse into enamel to degrade chromogen in order to reach whitening effect. The purpose for this study was to investigate tooth bleaching drug in human oral cells. The experiment is divided into in vitro and in vivo.In the in vitro part of the response at 1 and 8 hours, resulted in significantly decline in hNOK cell survival, ROS content, GSH, mitochondrial membrane potential .On the other hand, the LDH release,the oxidative damage of nuclear DNA, cell apoptosis will increase when the in 5 mM and 0.01 mM of H2O2, respectively. The copy number of mitochondrial showed no correlation with the concentration (1 hr, r =- 0.395; 8 hr, r =- 0.223).On the other hand, according to the spending time and the amount of the concentration,the correlation and the degree of mtDNA4977 become greater (1 hour, r = 0.3728; 8 hours, r = 0.873) . And the oxidative damage of nuclear DNA is also a positive correlation with mtDNA4977(1 hour,r =0.606；8 hour, r=0.863) In vivo study, 24 subjects use the teeth bleaching reagent for one month. And they wear the mouthguard that with the teeth bleaching reagent in 8 hours before sleeping daily.We obtain the mtDNA4977 have significant statistics(P < 0.05)from the first hour,the first day,the seventh day,the 14th day,and the 30th day between before and after using the teeth bleaching reagent.The 30th day after stop using the bleaching reagent is not statistically significant and the copy number are also not a statistically significant. Therefore, teeth bleaching reagent can cause on by oxidative stress human oral cell with various degrees, and even the impact of mitochondrial DNA changes. The future, we hope that the result of this study combined with clinical data. Not to harm a large number of injuries in the normal human oral cells, the pick to choose the appropriate concentration for teeth bleaching and the effect can be achieved and the scope to achieve security.
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