| 摘要: | 本研究利用rDNA限制酶片段(Amplified rDNA-restriction analysis, ARDRA)分析法分離鑑定出台灣人口腋下昀Corynebacterium屬(棒狀桿菌屬)細菌;再利用不同的萃取溶劑及萃取方式，針對天然中草進一步找尋天然切菌劑加以抑制其竺其生長。本研究採用80%乙醇及1,3丁二醇的兩種萃取溶劑，其中以80%乙醇萃取之中草藥萃取液的抑菌效果普遍優於1,3丁二醇萃取法。目前已篩選近五十種中草藥或天然成份中，以蘇鐵(抑菌圈17.0 mm).白芍(抑菌圈15.0 mm)、虎杖(抑菌圈15.0mm)、大黃(抑菌圈13.S mm)抑菌效果較為顯著。另外，本研究亦使用化粧品常用之抑菌劑-對蹕苯甲酸甲酯oropyl paraben, PP) 、二苯氧基乙醇(Phenoxyethanol)及殺菌劑三氯沙(Triclosan)來進行拙茵能力的比較。目前市售體味抑制產品成分之多為人工化學成分，有相關研究指出其副作用及致畸危險性，期望藉由本研究結果，開發出對人體無害的天然抑菌成份，以供體味抑制相關產品作多方面的應用。
In this study, amplified rDNA-restriction analysis (ARDRA) was applied to identif Corynebaclerium species isolated hom the axilla ofTaiwanese. On the other hand, by using different solvents and extraction methods, we tried to find natural extracts hom Chinese herbs possessing bactericidal activity for reducing axillary corynefonns. Firstly, we found that 80% ethanol is more effective than 1,3 butylene glycol as an extraction solvent. In addition, the extracts of four Chinese herbs exhibiting excellent antibacterial effects, including Lycas revolula Thunbs, Paeonia lactfora Pall, PoDgoni Cuspidati Rhizoma, and Rheum oficinale Baili, were discovered with the diameters of the clear inhibitoy zones of 17 mm, 15 mm, 15 mm, and 13.5 mm, respectively. Moreover, we also compared with the anti-coryneform activities ofthe routine antibacterials, methyl paraben (MP), propyl paraben (PP), phenoxyethanol, and triclosan, used in cosmetics. The antiperspirants and deodorants cunently used for odor control consist of chemical compositions and anificial presewatives, indicating that these products have several side effects and teratogenic risks shown in related studies. Therefore, we look forward to developing an innocuous natural product containing herbal extracts with the antibacterial capacity for the reduction of axillary malodor. |
