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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/22761

    標題: Evaluation Of the Non-Radioactive Detection of the CAG Repeats in the Machado-Joseph Disease Gene
    作者: Ming-Jiuan Wu
    Long-Wu Chou
    Ming-Eng Wang
    貢獻者: 食品衛生系
    關鍵字: Coagulation
    Membrane Mlcrofiltration
    triplet repeat
    日期: 1998
    上傳時間: 2010-05-11 14:09:04 (UTC+8)
    摘要: Machado-Joseph disease (MJD) is associated with the expansion of a CAG triplet repeat in a novel gene on 14q32.1. We evaluated a non-radioactive detection method for the screen of MJD. This non-radioactive PCR combined with agarose gel electrophoresis provides a comparably sensitive and accurate detectlon method as radioactive PCR wlth polyacrylamide gel electrophoresis. As low as 25 ng of genomic DNA can be speclfically amplified by PCR and the numbers of CAG repeat can be determined by agarose gel electrophoresis. This safe and quick detection method can by used in routine clinical laboratories for screening high-risk population of MJD.
    關聯: 嘉南學報 24期 : p.45-51
    Appears in Collections:[嘉南學報] 24 期 (1998)
    [ 食品科技系 ] 期刊論文

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