Potential of mean force of the hepatitis C virus core protein–monoclonal 19D9D6 antibody interaction

doi:10.1016/j.bpc.2009.09.004

CNU IR > College of Smart Living > Dept. of Information Management > Periodical Articles > Item 310902800/22598

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/22598

Title:	Potential of mean force of the hepatitis C virus core protein–monoclonal 19D9D6 antibody interaction
Authors:	Yeng-Tseng Wang Cheng-Lung Chen
Contributors:	資訊管理系
Keywords:	Molecular dynamics Potential of mean force Hepatitis C virus Antibody
Date:	2009-09
Issue Date:	2010-04-13 13:58:15 (UTC+8)
Abstract:	Antigen–antibody interactions are critical for understanding antigen–antibody associations in immunology. To shed further light on this que stion, we studied a dissociation of the 19D9D6-HCV core protein antibody complex structure. However, forced separations in single molecule experiments are difficult, and therefore molecular simulation techniqueswere applied in our study. The stretching, that is, the distance between the center of mass of theHCV core protein and the 19D9D6 antibody, has been studied using the potential ofmean force calculations based on molecular dynamics and the explicit water model. Our simulations indicate that the 7 residues Gly70, Gly72, Gly134, Gly158, Glu219, Gln221 and Tyr314, the interaction region (antibody), and the 14 interprotein molecular hydrogen bonds might play important roles in the antigen–antibody interaction, and this findingmay be useful for protein engineering of this antigen–antibody structure. In addition, the 3 residues Gly134, Gly158 and Tyr314 might be more important in the development of bioactive antibody analogs.
Relation:	Biophysical Chemistry 145：p.86–90
Appears in Collections:	[Dept. of Information Management] Periodical Articles

Files in This Item:

File	Size	Format
Potential of mean force of the hepatitis C virus core protein–monoclonal 19D9D6 antibody interaction .pdf	943Kb	Adobe PDF	1824	View/Open

Loading...