多酚化合物活性及有效成分探討 ─ 子計畫二：川陳皮素(nobiletin)及其代謝產物3',4'-dihydroxy-5,6,7,8-tetramethoxyflavone對LDL氧化及脂泡細胞形成之影響

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Title:	多酚化合物活性及有效成分探討 ─ 子計畫二：川陳皮素(nobiletin)及其代謝產物3',4'-dihydroxy-5,6,7,8-tetramethoxyflavone對LDL氧化及脂泡細胞形成之影響
Authors:	吳明娟
Contributors:	生物科技系(所)
Keywords:	low-density lipoprotein (LDL) atherogenesis scavenger receptor A (SR-A) CD36
Date:	2009
Issue Date:	2010-03-02 13:52:02 (UTC+8)
Publisher:	台南縣：嘉南藥理科技大學藥理學院
Abstract:	There is accumulating evidence that LDL oxidation is essential for atherogenesis, and antioxidants that prevent oxidation may either decelerate or reduce atherogenesis. Current study focused on the effect and mechanism of 3',4'-dihydroxy-5,6,7,8-tetramethoxyflavone (DTF), a major metabolite of nobiletin (NOB, a citrus polymethoxylated flavone) on atherogenesis. We found DTF had stronger inhibitory activity than α-tocopherol on inhibiting Cu2+-mediated LDL oxidation measured by thiobarbituric acid-reactive substances assay (TBARS), conjugated diene formation and electrophoretic mobility. Monocyte-to-macrophage differentiation plays a vital role in early atherogenesis. DTF (10-20 μM) dose-dependently attenuated differentiation along with the reduced gene expression of scavenger receptors, CD36 and SR-A, in both PMA- and oxidized low density lipoprotein (oxLDL)-stimulated THP-1 monocytes. Furthermore, DTF treatment of monocytes and macrophages lead to reduction of fluorescent DiI-acLDL and DiI-oxLDL uptake. In conclusion, at least three mechanisms are at work in parallel: DTF reduces LDL oxidation, attenuates monocyte differentiation into macrophage, and blunts uptake of modified LDL by macrophage. The effect is different from that of NOB, from which DTF is derived. This study thus significantly enhanced our understanding on how DTF may be beneficial against atherogenesis.
Relation:	計畫編號：CN9802
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Chna Project

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