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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/22208


    標題: Using High-Concentration Trypsin-Immobilized Magnetic Nanoparticles for Rapid In Situ Protein Digestion at Elevated Temperature
    作者: Jingyueh Jeng
    Mon-Fang Lin
    Fong-Yu Cheng
    Chen-Sheng Yeh
    Jentaie Shiea
    貢獻者: 生物科技系
    日期: 2007-08
    上傳時間: 2010-01-12 09:13:17 (UTC+8)
    摘要: We describe an innovative approach - using a high concentration of trypsin-modified magnetic nanoparticles (TMNPs) - for the rapid and efficient digestion of proteins at elevated temperature. The required digestion time could be reduced to less than 10 s. After digestion, the TMNPs were collected magnetically from the sample solution for reuse and the digested peptides were characterized using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Protein digestion was optimized when using the TMNPs (5 �g/�L) at 57�C; a significantly high peptide coverage was achieved for protein identification (e.g., 98% for lysozyme). Although a high concentration of TMNPs was used for digestion, the short digestion time led to much lower amounts of trypsin peptides being produced through self-digestion. As a result, interference in the mass spectrometric detection of the peptide ions was reduced significantly.
    關聯: Rapid Communications in Mass Spectrometry 21(18): p.3060-3068
    Appears in Collections:[生物科技系(所)] 期刊論文

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