Requirement of MMP-3 in anchorage-independent growth of oral squamous cell carcinomas.

doi:10.1111/j.1600-0714.2007.00524.x

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/22201

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/22201

Title:	Requirement of MMP-3 in anchorage-independent growth of oral squamous cell carcinomas.
Authors:	Shyun-Yeu Liu Young-Chau Liu Wen-Tsung Huang Guan-Cheng Huang Hsiao-Jing Su Mei-Huei Lin
Contributors:	生物科技系
Keywords:	esophageal carcinoma matrix metalloproteinase-3 oral squamous cell carcinoma stromelysin-1
Date:	2007-04
Issue Date:	2010-01-12 09:13:06 (UTC+8)
Abstract:	Background: Matrix metalloproteinase-3 (MMP-3) is expressed in various carcinomas; however, its function is not clearly established. This study was to assess its possible role in oral squamous cell carcinomas (OSCCs). Materials and methods: Specimens of seven oral pre-malignant lesions (OPMLs) and 92 OSCCs were subjected to MMP-3 detection by RT-PCR and Western blot. Antisense oligodeoxynucleotides (AODNs) of MMP-3 were used to transfect OSCC (OECM-1 and SCC-9) and esophageal carcinoma (CE81T/VGH) cell lines, and their growth was subsequently analyzed by XTT and soft-agar colony assay. Results: MMP-3 transcript was preferentially expressed in OSCCs (71 of 92, 77%) than in OPMLs (two of seven, 29%; P = 0.012). Both MMP-3 transcript and protein levels were significantly higher in OSCC masses than in neighboring tissues (P < 0.0001 and P = 0.04, respectively). Growth of the three cell lines was not affected, while the colony numbers of OECM-1 and CE81T/VGH were significantly reduced by the transfection of MMP-3 AODNs (P = 0.002 and P = 0.004, respectively). SCC-9 did not form colonies in soft-agar/medium. Conclusions: MMP-3 function may be required in most OSCCs, and it may support the anchorage-independent growth of both OSCC and esophageal carcinoma.
Relation:	Journal of Oral Pathology & Medicine 36(7)： p.430-435
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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