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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/22196


    Title: Identification of Phenolic Antioxidants from Sword Brake Fern (Pteris ensiformis Burm.)
    Authors: Yung-Husan Chen
    Fang-Rong Chang
    Yih-Jer Lin
    Lisu Wang
    Jinn-Fen Chen
    Yang-Chang Wu
    Ming-Jiuan Wu
    Contributors: 生物科技系
    Keywords: Pteris ensiformis
    DPPH
    Caffeic acid
    Hispidin
    TEAC
    Date: 2007
    Issue Date: 2010-01-12 09:12:58 (UTC+8)
    Publisher: Elsevier
    Abstract: Sword Brake fern (Pteris ensiformis Burm.) is one of the most common ingredients of traditional herbal drinks in Taiwan. In an effort to identify antioxidants from the aqueous extract of Sword Brake fern (SBF), the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity-guided isolation was employed. Three new compounds, kaempferol 3-O-α-l-rhamnopyranoside-7-O-[α-d-apiofuranosyl-(1-2)-β-d-glucopyranoside] (1), 7-O-caffeoylhydroxymaltol 3-O-β-d-glucopyranoside (3) and hispidin 4-O-β-d-glucopyranoside (4), together with five known compounds, kaempferol 3-O-α-l-rhamnopyranosid-7-O-β-d-glucopyranoside (2), caffeic acid (5), 5-O-caffeoylquinic acid (6), 3,5-di-O-caffeoylquinic acid (7) and 4,5-di-O-caffeoylquinic acid (8) were isolated and determined on the basis of spectroscopic analyses. HPLC with UV detector was further employed to analyze the content of each compound in SBF based on the retention time by comparison with isolated pure compounds. It was found that the most abundant phenolic compound was compound 3, followed by compounds 7 and 4. The di-O-caffeoylquinic acids (7 and 8) have the strongest DPPH scavenging potential with IC50 around 10 μM and the highest Trolox equivalent antioxidant capacity (TEAC) about 2 mM. This data indicates that SBF is rich in phenolic antioxidants.
    Relation: Food Chemistry 105(1) : p.48-56
    Appears in Collections:[Dept. of Biotechnology (including master's program)] Periodical Articles

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