Antiatherogenic Effects of Kaempferol and Rhamnocitrin

doi:10.1021/jf0717788

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標題:	Antiatherogenic Effects of Kaempferol and Rhamnocitrin
作者:	Yi-Chen Tu Tzi-Wei Lian Jui-Hung Yen Zong-Tsi Chen Ming-Jiuan Wu
貢獻者:	生物科技系醫藥化學系
關鍵字:	kaempferol rhamnocitrin low-density lipoprotein (LDL) atherogenesis class B scavenger receptor (CD36) Dil-oxLDL
日期:	2007-11
上傳時間:	2010-01-12 09:12:56 (UTC+8)
出版者:	Amer Chemical Soc
摘要:	Atherosclerosis is a chronic inflammatory disease of the arterial wall. Kaempferol and rhamnocitrin (kaempferol 7-O-methyl ether) are two anti-inflammatory flavonoids commonly found in plants. The aim of this study is to investigate the function of kaempferol and rhamnocitrin on prevention of atherosclerosis. Chemical analyses demonstrated that kaempferol and rhamnocitrin were scavengers of DPPH (1,1-diphenyl-2-picrylhydrazyl) with IC50 of 26.10 ± 1.33 and 28.38 ± 3.07 µM, respectively. Copper-induced low-density lipoprotein (LDL) oxidation was inhibited by kaempferol and rhamnocitrin, with similar potency, as measured by decreased formation of malondialdehyde and relative electrophoretic mobility (REM) on agarose gel, while rhamnocitrin reduced delayed formation of conjugated dienes better than kaempferol. Cholesterol-laden macrophages are the hallmark of atherogenesis. The class B scavenger receptor, CD36, binds oxidized low-density lipoprotein (oxLDL), is found in atherosclerotic lesions, and is up-regulated by oxLDL. Addition of kaempferol and rhamnocitrin (20 µM) caused significant reductions in cell surface CD36 protein expression in THP-1-derived macrophages (p < 0.05). Reverse transcription quantitative PCR (RT-Q-PCR) showed that kaempferol and rhamnocitrin (20 µM) decreased oxLDL-induced CD36 mRNA expression (p < 0.01 and p < 0.05, respectively). Kaempferol- and rhamnocitrin-treated macrophages also showed reduction in 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanide perchlorate (DiI)-labeled oxLDL uptake. Current evidences indicate that kaempferol and rhamnocitrin not only protect LDL from oxidation but also prevent atherogenesis through suppressing macrophage uptake of oxLDL.
關聯:	Journal of agricultural and food chemistry 55(24)： p.9969-9976
顯示於類別:	[生物科技系(所)] 期刊論文 [食藥產業暨檢測科技系(含五專)] 期刊論文

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